Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020774835> ?p ?o ?g. }
- W2020774835 endingPage "1375" @default.
- W2020774835 startingPage "1368" @default.
- W2020774835 abstract "ABSTRACT Reoviruses infect virtually all mammalian species, and infection of humans is associated with mild gastrointestinal or upper respiratory illnesses. To improve reovirus detection strategies, we developed a reverse transcription-PCR technique to amplify a fragment of the reovirus L1 gene segment. This assay was capable of detecting 44 of 44 reovirus field isolate strains and was sufficiently sensitive to detect nearly a single viral particle (1.16 ± 0.13) per PCR of prototype strain type 3 Dearing. Pairwise comparisons of the 44 partial L1 gene sequences revealed that nucleotide variability ranged from 0 to 24.7%, with most of the nucleotide polymorphism occurring at synonymous positions. Phylogenetic trees generated from amplified L1 gene sequences suggest that multiple alleles of the L1 gene cocirculate in nature and that genetic diversity of the L1 gene is largely independent of the host species, geographic locale, or date of isolation. Phylogenetic trees constructed from the L1 gene sequences are distinct from those constructed from the four reovirus S-class gene segments, which supports the hypothesis that reovirus gene segments reassort in nature. This study establishes a new sensitive and specific technique for the identification of mammalian reoviruses and enhances our understanding of reovirus evolution." @default.
- W2020774835 created "2016-06-24" @default.
- W2020774835 creator A5023660486 @default.
- W2020774835 creator A5023812277 @default.
- W2020774835 creator A5026502819 @default.
- W2020774835 creator A5034999570 @default.
- W2020774835 creator A5035414920 @default.
- W2020774835 creator A5045018661 @default.
- W2020774835 creator A5059052635 @default.
- W2020774835 creator A5065007450 @default.
- W2020774835 date "2002-04-01" @default.
- W2020774835 modified "2023-10-18" @default.
- W2020774835 title "Detection of Mammalian Reovirus RNA by Using Reverse Transcription-PCR: Sequence Diversity within the λ3-Encoding L1 Gene" @default.
- W2020774835 cites W1507590754 @default.
- W2020774835 cites W1509298701 @default.
- W2020774835 cites W1561071283 @default.
- W2020774835 cites W1574028564 @default.
- W2020774835 cites W1574770313 @default.
- W2020774835 cites W1592889735 @default.
- W2020774835 cites W1600760304 @default.
- W2020774835 cites W1601081247 @default.
- W2020774835 cites W1812830413 @default.
- W2020774835 cites W1876662144 @default.
- W2020774835 cites W1952957089 @default.
- W2020774835 cites W1972160946 @default.
- W2020774835 cites W1978396569 @default.
- W2020774835 cites W1978548761 @default.
- W2020774835 cites W1978778887 @default.
- W2020774835 cites W1979378596 @default.
- W2020774835 cites W1990441598 @default.
- W2020774835 cites W1996465772 @default.
- W2020774835 cites W2002696097 @default.
- W2020774835 cites W2021119916 @default.
- W2020774835 cites W2033158357 @default.
- W2020774835 cites W2034124714 @default.
- W2020774835 cites W2037859614 @default.
- W2020774835 cites W2080183724 @default.
- W2020774835 cites W2083184265 @default.
- W2020774835 cites W2098251708 @default.
- W2020774835 cites W2101538335 @default.
- W2020774835 cites W2121864361 @default.
- W2020774835 cites W2128923561 @default.
- W2020774835 cites W2135880580 @default.
- W2020774835 cites W4253838422 @default.
- W2020774835 doi "https://doi.org/10.1128/jcm.40.4.1368-1375.2002" @default.
- W2020774835 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/140344" @default.
- W2020774835 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11923358" @default.
- W2020774835 hasPublicationYear "2002" @default.
- W2020774835 type Work @default.
- W2020774835 sameAs 2020774835 @default.
- W2020774835 citedByCount "43" @default.
- W2020774835 countsByYear W20207748352012 @default.
- W2020774835 countsByYear W20207748352013 @default.
- W2020774835 countsByYear W20207748352014 @default.
- W2020774835 countsByYear W20207748352015 @default.
- W2020774835 countsByYear W20207748352016 @default.
- W2020774835 countsByYear W20207748352017 @default.
- W2020774835 countsByYear W20207748352018 @default.
- W2020774835 countsByYear W20207748352019 @default.
- W2020774835 countsByYear W20207748352020 @default.
- W2020774835 countsByYear W20207748352021 @default.
- W2020774835 countsByYear W20207748352022 @default.
- W2020774835 countsByYear W20207748352023 @default.
- W2020774835 crossrefType "journal-article" @default.
- W2020774835 hasAuthorship W2020774835A5023660486 @default.
- W2020774835 hasAuthorship W2020774835A5023812277 @default.
- W2020774835 hasAuthorship W2020774835A5026502819 @default.
- W2020774835 hasAuthorship W2020774835A5034999570 @default.
- W2020774835 hasAuthorship W2020774835A5035414920 @default.
- W2020774835 hasAuthorship W2020774835A5045018661 @default.
- W2020774835 hasAuthorship W2020774835A5059052635 @default.
- W2020774835 hasAuthorship W2020774835A5065007450 @default.
- W2020774835 hasBestOaLocation W20207748351 @default.
- W2020774835 hasConcept C104317684 @default.
- W2020774835 hasConcept C159047783 @default.
- W2020774835 hasConcept C193252679 @default.
- W2020774835 hasConcept C2522874641 @default.
- W2020774835 hasConcept C2779416561 @default.
- W2020774835 hasConcept C2781367151 @default.
- W2020774835 hasConcept C54355233 @default.
- W2020774835 hasConcept C86803240 @default.
- W2020774835 hasConcept C90132467 @default.
- W2020774835 hasConceptScore W2020774835C104317684 @default.
- W2020774835 hasConceptScore W2020774835C159047783 @default.
- W2020774835 hasConceptScore W2020774835C193252679 @default.
- W2020774835 hasConceptScore W2020774835C2522874641 @default.
- W2020774835 hasConceptScore W2020774835C2779416561 @default.
- W2020774835 hasConceptScore W2020774835C2781367151 @default.
- W2020774835 hasConceptScore W2020774835C54355233 @default.
- W2020774835 hasConceptScore W2020774835C86803240 @default.
- W2020774835 hasConceptScore W2020774835C90132467 @default.
- W2020774835 hasIssue "4" @default.
- W2020774835 hasLocation W20207748351 @default.
- W2020774835 hasLocation W20207748352 @default.
- W2020774835 hasLocation W20207748353 @default.
- W2020774835 hasOpenAccess W2020774835 @default.
- W2020774835 hasPrimaryLocation W20207748351 @default.
- W2020774835 hasRelatedWork W1970732127 @default.