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- W2020809711 abstract "The combination of proteomic approach and affinity chromatography (GSTrap™ HP) has been used to identify and investigate the differential expression of GSTs under toxic challenge and during the course of development in Aedes albopictus. There were multiple isoforms of glutathione transferases (GSTs) expressed in the three developmental stages of Aedes albopictus. On a 2D gel map, the results indicate that there were 19 spots with a molecular mass in the range of 24–30 kDa. Three types of GSTs were identified from the spots; namely GSTS1, GSTD1, and GST Theta. Spots identified as GSTS1 and GSTD1 were further designated as GSTS1–1 to GSTS1–5 and GSTD1–1 to GSTD1–13 respectively. Isoforms GSTD1–10, GSTD1–11, GSTD1–12, GSTD1–13, and GSTT were absent in the adult life stage. Isoenzymes GSTS1–3, GSTD1–2, and GSTD1–6 appeared as the major expressed isoforms in all the life stages. The effect of free radical generators such as hydrogen peroxide and paraquat on the expression of the GST isoforms in larvae mosquitoes was also investigated. Isoforms GSTD1–1, GSTD1–4, GSTS1–4, and GSTD1–12 had > 1.5 fold increase when the larvae were challenged with both chemicals. GSTS1–3, GSTS1–4, GSTD1–1, GSTD1–2, GSTD1–3, GSTD1–6, and GSTD1–12 were significantly expressed (p < 0.05) with > 1.5 fold change when the larvae were challenged with hydrogen peroxide. On the contrary, different GST isoforms were responsive towards paraquat challenge. Those isoforms were GSTS1–2, GSTS1–4, GSTD1–1, GSTD1–5, GSTD1–9, GSTD1–11, GSTD1–12, and GSTT (with > 1.5 fold change). The purified larvae GSTs seemed to have limited substrate specificities with the ability to only conjugate 1-chloro-2,4-dinitrobenzene (CDNB) and 1,2-dichloro-4-nitrobenzene (DCNB). The GSTs have however shown that they were capable of detoxifying lipid peroxidation products and possessing glutathione peroxidase activities." @default.
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- W2020809711 date "1995-08-01" @default.
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- W2020809711 title "The effect of asperatam on the spontsneous and induced clastogenesls in mice" @default.
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