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- W2020836699 abstract "Eleven mares were induced to ovulate by treatment with 3,000 iu hCG and subsequently inseminated with frozen-thawed semen. The time of ovulation was determined by transrectal ultrasonography of the ovaries performed every 4 h. At different times after ovulation ova were collected from oviducts removed by surgery through a flank incision under general anaesthesia. The presumed fertilised ova were cultured for 20 min in a medium containing [3H]uridine, fixed, embedded in Epon, semithin-sectioned and processed for autoradiography. Selected semithin sections were re-embedded and processed for transmission electron microscopy. Spherical, paternal and maternal pronuclei were observed within 12 h after ovulation, and by 19 h the pronuclei had migrated to close apposition. The 2-cell stage was seen within 34 h and a major activation of RNA synthesis was detected at 64 h after ovulation in 4 blastomeres of a 6-cell embryo, suggesting maternal-embryonic transition apparently occurs at the 4th cell cycle." @default.
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- W2020836699 date "1993-10-01" @default.
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- W2020836699 title "In-vivo fertilisation and initial embryogenesis in the mare" @default.
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