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• W2020846102 abstract "This study compares the human immune systems of neonates and adults. Flow cytometric analysis was used to study the cellular phenotypes of cord blood (CB) and adult peripheral blood (APB). Luminex analysis was used to determine the levels of cytokines in cell culture supernatants. Our findings indicate that T cells in CB were mainly naïve and thus less responsive to PMA/ionomycin with the synthesis of cytokines. The percentages of CD3+CD4+CD25high and of CD3+CD4+CD25dim cells expressing chemokine receptors were different between CB and APB. TLR1, TLR6 and TLR9 expressions on NK and NKT cells also differed between CB and APB. CB monocyte-derived macrophages responded better than APB macrophages to TLR ligands with increased secretion of inflammatory cytokines, especially IL-6. The high levels of the inflammatory cytokines in cell culture supernatants of CB were mainly due to higher numbers of responsive macrophages, since dendritic cell numbers were lower in CB than APB." @default.
• W2020846102 created "2016-06-24" @default.
• W2020846102 creator A5053216437 @default.
• W2020846102 creator A5071318619 @default.
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• W2020846102 date "2014-10-01" @default.
• W2020846102 modified "2023-09-25" @default.
• W2020846102 title "Naïve T cells, unconventional NK and NKT cells, and highly responsive monocyte-derived macrophages characterize human cord blood" @default.
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• W2020846102 doi "https://doi.org/10.1016/j.imbio.2014.06.001" @default.
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