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- W202086957 abstract "This chapter discusses the large-scale isolation of anucleated yeast protoplasts and their partial biochemical characterization. The procedure for isolation of anucleated yeast protoplasts by means of density gradient centrifugation allows obtaining relatively homogeneous preparations of anucleated protoplasts in yields sufficient for their biochemical characterization. The isolated nucleus-free protoplasts may be, in some cases, contaminated by remnants of the cell walls not completely dissolved during the protoplasting procedure. To avoid this, the time of incubation of yeast cells with the snail enzyme should be prolonged until no cell walls remain in the incubation mixture. The yield of anucleated protoplasts depends on the growth phase of the yeast used: it is higher with cells from the end of the exponential phase of growth when there is a high abundance of cells with small buds in the culture." @default.
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- W202086957 date "1985-01-01" @default.
- W202086957 modified "2023-09-26" @default.
- W202086957 title "ANUCLEATED YEAST PROTOPLASTS AND THEIR INABILITY TO REGENERATE THE CELL WALL" @default.
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- W202086957 doi "https://doi.org/10.1016/b978-0-12-428580-4.50056-3" @default.
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