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• W2020898319 startingPage "334" @default.
• W2020898319 abstract "Vascular calcification was considered to be a passive, degenerative, and end-stage process of vascular disease. However, bone associated proteins such as bone morphogenetic proteins, osteopontin, osteonectin, osteocalcin, and matrix Gla protein (MGP) have been found in the calcified atherosclerotic lesions. We studied by microarray analysis whether intact tissue and carotid plaque from the same patient differ in transcriptional profiling in response to arterial calcification.mRNA gene expression was measured by an Affymetrix GeneChip Human Gene 1.0 ST arrays (Affymetrix, Santa Clara, CA, USA) using RNA prepared from 68 specimens of endarterectomy from 34 patients.Integrin-binding sialoprotein (IBSP) was found to be differentially expressed. IBSP mRNA is over expressed in atheroma plaque (3.74 fold, p = 1.41E-09) in an intraindividual comparison. Besides, Carbonic anhydrase II (CA2) which known to be a putative calcification inhibitory molecule is over expressed more than 1.7 fold in carotid plaque (p = 1.26E-06).Although further evidence is needed, our results support previously available data. To our knowledge, this is the first report comparing gene expression between intact arterial tissue and carotid plaque using microarray analysis in order to identify calcification related genes. We suggest that plaques show a more pronounced induction of IBSP that may cause arterial calcification." @default.
• W2020898319 created "2016-06-24" @default.
• W2020898319 creator A5039175304 @default.
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• W2020898319 date "2012-12-01" @default.
• W2020898319 modified "2023-10-16" @default.
• W2020898319 title "Microarray analysis reveals overexpression of IBSP in human carotid plaques" @default.
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• W2020898319 doi "https://doi.org/10.2478/v10039-012-0056-0" @default.
• W2020898319 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23314561" @default.
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