FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020912981> ?p ?o ?g. }

• W2020912981 endingPage "12660" @default.
• W2020912981 startingPage "12655" @default.
• W2020912981 abstract "The last unidentified gene encoding an enzyme involved in ergosterol biosynthesis in Saccharomyces cerevisiae has been cloned. This gene, designated ERG27, encodes the 3-keto sterol reductase, which, in concert with the C-4 sterol methyloxidase (ERG25) and the C-3 sterol dehydrogenase (ERG26), catalyzes the sequential removal of the two methyl groups at the sterol C-4 position. We developed a strategy to isolate a mutant deficient in converting 3-keto to 3-hydroxy-sterols. An ergosterol auxotroph unable to synthesize sterol or grow without sterol supplementation was mutagenized. Colonies were then selected that were nystatin-resistant in the presence of 3-ketoergostadiene and cholesterol. A new ergosterol auxotroph unable to grow on 3-ketosterols without the addition of cholesterol was isolated. The gene (YLR100w) was identified by complementation. Segregants containing the YLR100w disruption failed to grow on various types of 3-keto sterol substrates. Surprisingly, when erg27 was grown on cholesterol- or ergosterol-supplemented media, the endogenous compounds that accumulated were noncyclic sterol intermediates (squalene, squalene epoxide, and squalene dioxide), and there was little or no accumulation of lanosterol or 3-ketosterols. Feeding experiments in which erg27 strains were supplemented with lanosterol (an upstream intermediate of the C-4 demethylation process) and cholesterol (an end-product sterol) demonstrated accumulation of four types of 3-keto sterols identified by GC/MS and chromatographic properties: 4-methyl-zymosterone, zymosterone, 4-methyl-fecosterone, and ergosta-7,24 (28)-dien-3-one. In addition, a fifth intermediate was isolated and identified by (1)H NMR as a 4-methyl-24, 25-epoxy-cholesta-7-en-3-one. Implications of these results are discussed." @default.
• W2020912981 created "2016-06-24" @default.
• W2020912981 creator A5002134442 @default.
• W2020912981 creator A5027241044 @default.
• W2020912981 creator A5028038671 @default.
• W2020912981 creator A5049346514 @default.
• W2020912981 creator A5065765152 @default.
• W2020912981 creator A5071424020 @default.
• W2020912981 creator A5086686038 @default.
• W2020912981 date "1999-10-26" @default.
• W2020912981 modified "2023-09-25" @default.
• W2020912981 title "Characterization of the <i>Saccharomyces cerevisiae ERG27</i> gene encoding the 3-keto reductase involved in C-4 sterol demethylation" @default.
• W2020912981 cites W1500619872 @default.
• W2020912981 cites W1518655990 @default.
• W2020912981 cites W1545800347 @default.
• W2020912981 cites W1966884806 @default.
• W2020912981 cites W1982050826 @default.
• W2020912981 cites W1992325956 @default.
• W2020912981 cites W1998809465 @default.
• W2020912981 cites W2006472852 @default.
• W2020912981 cites W2032439358 @default.
• W2020912981 cites W2036987057 @default.
• W2020912981 cites W204228673 @default.
• W2020912981 cites W2042986148 @default.
• W2020912981 cites W2052562460 @default.
• W2020912981 cites W2058193911 @default.
• W2020912981 cites W2066685853 @default.
• W2020912981 cites W2069350228 @default.
• W2020912981 cites W2074156945 @default.
• W2020912981 cites W2074378344 @default.
• W2020912981 cites W2080408951 @default.
• W2020912981 cites W2094489008 @default.
• W2020912981 cites W2145699815 @default.
• W2020912981 cites W2152311269 @default.
• W2020912981 cites W2167621347 @default.
• W2020912981 cites W2169333620 @default.
• W2020912981 cites W2188516802 @default.
• W2020912981 cites W2300552860 @default.
• W2020912981 cites W3305402 @default.
• W2020912981 cites W4238137301 @default.
• W2020912981 doi "https://doi.org/10.1073/pnas.96.22.12655" @default.
• W2020912981 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/23033" @default.
• W2020912981 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10535978" @default.
• W2020912981 hasPublicationYear "1999" @default.
• W2020912981 type Work @default.
• W2020912981 sameAs 2020912981 @default.
• W2020912981 citedByCount "91" @default.
• W2020912981 countsByYear W20209129812012 @default.
• W2020912981 countsByYear W20209129812014 @default.
• W2020912981 countsByYear W20209129812015 @default.
• W2020912981 countsByYear W20209129812016 @default.
• W2020912981 countsByYear W20209129812017 @default.
• W2020912981 countsByYear W20209129812018 @default.
• W2020912981 countsByYear W20209129812019 @default.
• W2020912981 countsByYear W20209129812020 @default.
• W2020912981 countsByYear W20209129812021 @default.
• W2020912981 countsByYear W20209129812022 @default.
• W2020912981 countsByYear W20209129812023 @default.
• W2020912981 crossrefType "journal-article" @default.
• W2020912981 hasAuthorship W2020912981A5002134442 @default.
• W2020912981 hasAuthorship W2020912981A5027241044 @default.
• W2020912981 hasAuthorship W2020912981A5028038671 @default.
• W2020912981 hasAuthorship W2020912981A5049346514 @default.
• W2020912981 hasAuthorship W2020912981A5065765152 @default.
• W2020912981 hasAuthorship W2020912981A5071424020 @default.
• W2020912981 hasAuthorship W2020912981A5086686038 @default.
• W2020912981 hasBestOaLocation W20209129811 @default.
• W2020912981 hasConcept C104317684 @default.
• W2020912981 hasConcept C134651460 @default.
• W2020912981 hasConcept C143065580 @default.
• W2020912981 hasConcept C150194340 @default.
• W2020912981 hasConcept C181199279 @default.
• W2020912981 hasConcept C190727270 @default.
• W2020912981 hasConcept C202199734 @default.
• W2020912981 hasConcept C20950039 @default.
• W2020912981 hasConcept C2776703722 @default.
• W2020912981 hasConcept C2777576037 @default.
• W2020912981 hasConcept C2777910421 @default.
• W2020912981 hasConcept C2778163477 @default.
• W2020912981 hasConcept C2778718757 @default.
• W2020912981 hasConcept C2779969316 @default.
• W2020912981 hasConcept C553450214 @default.
• W2020912981 hasConcept C55493867 @default.
• W2020912981 hasConcept C85411794 @default.
• W2020912981 hasConcept C86803240 @default.
• W2020912981 hasConceptScore W2020912981C104317684 @default.
• W2020912981 hasConceptScore W2020912981C134651460 @default.
• W2020912981 hasConceptScore W2020912981C143065580 @default.
• W2020912981 hasConceptScore W2020912981C150194340 @default.
• W2020912981 hasConceptScore W2020912981C181199279 @default.
• W2020912981 hasConceptScore W2020912981C190727270 @default.
• W2020912981 hasConceptScore W2020912981C202199734 @default.
• W2020912981 hasConceptScore W2020912981C20950039 @default.
• W2020912981 hasConceptScore W2020912981C2776703722 @default.
• W2020912981 hasConceptScore W2020912981C2777576037 @default.
• W2020912981 hasConceptScore W2020912981C2777910421 @default.
• W2020912981 hasConceptScore W2020912981C2778163477 @default.
• W2020912981 hasConceptScore W2020912981C2778718757 @default.

• next