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• W2020932026 abstract "Protein A bound to particles of colloidal gold is widely used for cytochemical localization of antigens. A method for detecting immune complexes on Western blots with protein A-gold has been developed. It involved the following steps: (i) electrophoretic separation of proteins, (ii) transfer to nitrocellulose, (iii) incubation with antibodies, and (iv) staining with protein A-gold. The amount of antigen detectable was 10-50 ng. The sensitivity could be improved by subsequent photochemical silver staining (1-10 ng detected), and was then comparable to autoradiograms using iodinated protein A." @default.
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• W2020932026 date "1984-10-01" @default.
• W2020932026 modified "2023-09-25" @default.
• W2020932026 title "“Golden blot”—Detection of polyclonal and monoclonal antibodies bound to antigens on nitrocellulose by protein A-gold complexes" @default.
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• W2020932026 doi "https://doi.org/10.1016/0003-2697(84)90518-9" @default.
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