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• W2020952022 abstract "ObjectiveTo assess the duration of the second and third cell cycles (cc) as predictors of blastocyst formation and implantation (I).DesignRetrospective cohort study.Materials and MethodsMonitoring of 6970 embryos revealed that 3107developed into blastocysts (B) and 3863 did not (NB). After transfer, 328 embryos were designated as 100%, when the number of gestational sacs matches with the transferred B or, 0%, when no chemical gestation was achieved. Timing of cleavage (hrs post-ICSI) from 2 to 8 cells (t2, t3, t4, t5, t6, t7, t8) was determined by time-lapse imaging. The following variables were studied: cc2a: cc length of the 1st blastomere to cleave from 2 to 3 cells; cc2b: cc length of the 2nd blastomere to cleave from 2 to 4 cells; cc3a: b, c, d: 3rd cc duration of the blastomere that cleaves to 5, 6, 7 and 8 cells; acc3: average cc3 (cc3a+cc3b+cc3c+cc3d)/4; blastomere synchrony in cleavage at cc2 (SR2= cc2a/cc2b) and cc3 (SR3=acc3/(t8-t3)).ResultsTabled 1ResultsMean ± SD according to B (95CI)In/out of OR (%B)ODS Ratio ORMean± SD according to I (95CI)In/out of OR (%I)ODS Ratio ORCc2aNB: 9.8±0.1 (9.6-10.0)/B: 10.5±3.9 (10.4-10.7)9.76-12.7 (54.9)/Out (32.6%)1.60: 10.9±3.6 (10.5-11.2)/100: 10.5±3.5 (10.2-10.9)Cc2bNB: 13.1±6.6 (12.9-13.3)/B: 12.3±3.9 (12.2-12.4)11.3-14.3 (51.6)/Out (36.1%)1.40: 12.4±3.6 (12.1-12.7)/100: 12.0±3.3 (11.7-12.3)RS2NB: 0.7±0.3 (0.7-0.8)/B: 0.9±0.2 (0.8-0.9)≤80.5 (28.7)/>80.5 (49.7)0.80: 0.9±0.2 (0.8-0.9)/100: 0.9±0.2 (0.8-0.9)Cc3aNB: 13.4±8.2 (13.1-13.7)/B: 13.6±5.6 (13.4-13.8)11.5-16.5 (55.5)/Out (39.8)1.30: 13.9±4.8 (13.4-14.3)/100: 13.6±4.3 (13.1-14.0)Cc3bNB:17.7±8.4 (17.4-18.0)/B: 16.5±5.8 (16.3-16.7)0: 16.3±4.8 (15.8-16.7)/100: 15.6±4.6 (15.2-16.1)Cc3cNB: 19.3±9.2 (18.9-19.6)/B: 17.8±6.7 (17.6-18.0)0: 17.9±6.0 (17.3-18.4)/100: 16.5±4.9 (16.0-17.0)<21.0 (48.0)/Out (34.1)1.7Cc3dNB: 23.5±10.6 (23.0-23.9)/B: 21.8±8.9 (21.5-22.2)0: 21.6±7.9 (20.8-22.4)/100: 19.9±7.3 (19.1-20.7)≤15.7 (54.4)/Out (43.2)1.4acc3NB: 17.6±6.5 (17.3-17.8)/B: 17.1±5.2 (16.9-17.3)0: 17.2±4.8 (16.7-17.7)/100: 16.1±4.4 (15.6-16.6)<18.9 (48.5)/Out (38.1)RS3NB: 0.7±0.2 (0.7-0.7)/B: 0.7±0.1 (0.7-0.8)≤0.7 (47.6)/>0.7 (63.5)1.50: 0.7±0.1 (0.7-0.9)/100: 0.8±0.1 (0.7-0.8) Open table in a new tab Conclusioncc2 and cc3 are associated with development to B and I, respectively. ObjectiveTo assess the duration of the second and third cell cycles (cc) as predictors of blastocyst formation and implantation (I). To assess the duration of the second and third cell cycles (cc) as predictors of blastocyst formation and implantation (I). DesignRetrospective cohort study. Retrospective cohort study. Materials and MethodsMonitoring of 6970 embryos revealed that 3107developed into blastocysts (B) and 3863 did not (NB). After transfer, 328 embryos were designated as 100%, when the number of gestational sacs matches with the transferred B or, 0%, when no chemical gestation was achieved. Timing of cleavage (hrs post-ICSI) from 2 to 8 cells (t2, t3, t4, t5, t6, t7, t8) was determined by time-lapse imaging. The following variables were studied: cc2a: cc length of the 1st blastomere to cleave from 2 to 3 cells; cc2b: cc length of the 2nd blastomere to cleave from 2 to 4 cells; cc3a: b, c, d: 3rd cc duration of the blastomere that cleaves to 5, 6, 7 and 8 cells; acc3: average cc3 (cc3a+cc3b+cc3c+cc3d)/4; blastomere synchrony in cleavage at cc2 (SR2= cc2a/cc2b) and cc3 (SR3=acc3/(t8-t3)). Monitoring of 6970 embryos revealed that 3107developed into blastocysts (B) and 3863 did not (NB). After transfer, 328 embryos were designated as 100%, when the number of gestational sacs matches with the transferred B or, 0%, when no chemical gestation was achieved. Timing of cleavage (hrs post-ICSI) from 2 to 8 cells (t2, t3, t4, t5, t6, t7, t8) was determined by time-lapse imaging. The following variables were studied: cc2a: cc length of the 1st blastomere to cleave from 2 to 3 cells; cc2b: cc length of the 2nd blastomere to cleave from 2 to 4 cells; cc3a: b, c, d: 3rd cc duration of the blastomere that cleaves to 5, 6, 7 and 8 cells; acc3: average cc3 (cc3a+cc3b+cc3c+cc3d)/4; blastomere synchrony in cleavage at cc2 (SR2= cc2a/cc2b) and cc3 (SR3=acc3/(t8-t3)). ResultsTabled 1ResultsMean ± SD according to B (95CI)In/out of OR (%B)ODS Ratio ORMean± SD according to I (95CI)In/out of OR (%I)ODS Ratio ORCc2aNB: 9.8±0.1 (9.6-10.0)/B: 10.5±3.9 (10.4-10.7)9.76-12.7 (54.9)/Out (32.6%)1.60: 10.9±3.6 (10.5-11.2)/100: 10.5±3.5 (10.2-10.9)Cc2bNB: 13.1±6.6 (12.9-13.3)/B: 12.3±3.9 (12.2-12.4)11.3-14.3 (51.6)/Out (36.1%)1.40: 12.4±3.6 (12.1-12.7)/100: 12.0±3.3 (11.7-12.3)RS2NB: 0.7±0.3 (0.7-0.8)/B: 0.9±0.2 (0.8-0.9)≤80.5 (28.7)/>80.5 (49.7)0.80: 0.9±0.2 (0.8-0.9)/100: 0.9±0.2 (0.8-0.9)Cc3aNB: 13.4±8.2 (13.1-13.7)/B: 13.6±5.6 (13.4-13.8)11.5-16.5 (55.5)/Out (39.8)1.30: 13.9±4.8 (13.4-14.3)/100: 13.6±4.3 (13.1-14.0)Cc3bNB:17.7±8.4 (17.4-18.0)/B: 16.5±5.8 (16.3-16.7)0: 16.3±4.8 (15.8-16.7)/100: 15.6±4.6 (15.2-16.1)Cc3cNB: 19.3±9.2 (18.9-19.6)/B: 17.8±6.7 (17.6-18.0)0: 17.9±6.0 (17.3-18.4)/100: 16.5±4.9 (16.0-17.0)<21.0 (48.0)/Out (34.1)1.7Cc3dNB: 23.5±10.6 (23.0-23.9)/B: 21.8±8.9 (21.5-22.2)0: 21.6±7.9 (20.8-22.4)/100: 19.9±7.3 (19.1-20.7)≤15.7 (54.4)/Out (43.2)1.4acc3NB: 17.6±6.5 (17.3-17.8)/B: 17.1±5.2 (16.9-17.3)0: 17.2±4.8 (16.7-17.7)/100: 16.1±4.4 (15.6-16.6)<18.9 (48.5)/Out (38.1)RS3NB: 0.7±0.2 (0.7-0.7)/B: 0.7±0.1 (0.7-0.8)≤0.7 (47.6)/>0.7 (63.5)1.50: 0.7±0.1 (0.7-0.9)/100: 0.8±0.1 (0.7-0.8) Open table in a new tab Conclusioncc2 and cc3 are associated with development to B and I, respectively. cc2 and cc3 are associated with development to B and I, respectively." @default.
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• W2020952022 title "Exploring new morphokinetic variables of in vitro and in vivo embryo development" @default.
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