Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020961504> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2020961504 endingPage "157" @default.
- W2020961504 startingPage "151" @default.
- W2020961504 abstract "Ricin is a potent plant toxin consisting of two disulfide-bonded subunits. The A chain of ricin is an N-glycosidase which inactivates 28 S RNA and inhibits protein synthesis. The B chain is a galactose-specific lectin with two galactose-binding sites. The genes encoding preproricin and its A and B chains have been cloned and expressed. In addition, X-ray crystallographic studies have identified the galactose-contact residues in both the high- and low-affinity galactose-binding sites of the B chain. In this study, the high-affinity galactose-contact residue of the B chain was changed from Asn-255 to Ala-255 by oligonucleotide-directed mutagenesis. The resulting mutant was sequenced to confirm the presence of a single mutation and was expressed in Cos-M6 cells. Both wild-type and mutant recombinant B chain could be immunoprecipitated with a heterologous anti-B chain antibody and both could form A-B heterodimers. However, as compared to the wild-type, the mutant B chain lacked more than 99% of its lectin activity and cytotoxicity as an A-B dimer. In conclusion, altering the contact residue of the high-affinity galactose-binding site of ricin B chain from Asn-255 to Ala-255 abrogates more than 99% of its lectin activity and the cytotoxicity of the A-B heterodimer to ricin-sensitive cells." @default.
- W2020961504 created "2016-06-24" @default.
- W2020961504 creator A5008015987 @default.
- W2020961504 creator A5084475433 @default.
- W2020961504 date "1990-06-01" @default.
- W2020961504 modified "2023-09-23" @default.
- W2020961504 title "Expression and functional properties of genetically engineered ricin B chain lacking galactose-binding activity" @default.
- W2020961504 cites W1482431053 @default.
- W2020961504 cites W1500247723 @default.
- W2020961504 cites W1518889327 @default.
- W2020961504 cites W1530321233 @default.
- W2020961504 cites W1586747665 @default.
- W2020961504 cites W159370705 @default.
- W2020961504 cites W1918119607 @default.
- W2020961504 cites W1965461790 @default.
- W2020961504 cites W1969757078 @default.
- W2020961504 cites W1980238668 @default.
- W2020961504 cites W1985697463 @default.
- W2020961504 cites W1989665007 @default.
- W2020961504 cites W1994624380 @default.
- W2020961504 cites W2043120873 @default.
- W2020961504 cites W2067147862 @default.
- W2020961504 cites W2075077207 @default.
- W2020961504 cites W2084884863 @default.
- W2020961504 cites W2086193113 @default.
- W2020961504 cites W2089598244 @default.
- W2020961504 cites W2149866422 @default.
- W2020961504 cites W295780 @default.
- W2020961504 doi "https://doi.org/10.1016/0167-4781(90)90035-z" @default.
- W2020961504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1694694" @default.
- W2020961504 hasPublicationYear "1990" @default.
- W2020961504 type Work @default.
- W2020961504 sameAs 2020961504 @default.
- W2020961504 citedByCount "21" @default.
- W2020961504 countsByYear W20209615042017 @default.
- W2020961504 countsByYear W20209615042021 @default.
- W2020961504 countsByYear W20209615042022 @default.
- W2020961504 crossrefType "journal-article" @default.
- W2020961504 hasAuthorship W2020961504A5008015987 @default.
- W2020961504 hasAuthorship W2020961504A5084475433 @default.
- W2020961504 hasConcept C103408237 @default.
- W2020961504 hasConcept C104317684 @default.
- W2020961504 hasConcept C143065580 @default.
- W2020961504 hasConcept C153911025 @default.
- W2020961504 hasConcept C16318435 @default.
- W2020961504 hasConcept C185592680 @default.
- W2020961504 hasConcept C2777200438 @default.
- W2020961504 hasConcept C2777367657 @default.
- W2020961504 hasConcept C2778173381 @default.
- W2020961504 hasConcept C2780758401 @default.
- W2020961504 hasConcept C55493867 @default.
- W2020961504 hasConcept C86803240 @default.
- W2020961504 hasConceptScore W2020961504C103408237 @default.
- W2020961504 hasConceptScore W2020961504C104317684 @default.
- W2020961504 hasConceptScore W2020961504C143065580 @default.
- W2020961504 hasConceptScore W2020961504C153911025 @default.
- W2020961504 hasConceptScore W2020961504C16318435 @default.
- W2020961504 hasConceptScore W2020961504C185592680 @default.
- W2020961504 hasConceptScore W2020961504C2777200438 @default.
- W2020961504 hasConceptScore W2020961504C2777367657 @default.
- W2020961504 hasConceptScore W2020961504C2778173381 @default.
- W2020961504 hasConceptScore W2020961504C2780758401 @default.
- W2020961504 hasConceptScore W2020961504C55493867 @default.
- W2020961504 hasConceptScore W2020961504C86803240 @default.
- W2020961504 hasIssue "2" @default.
- W2020961504 hasLocation W20209615041 @default.
- W2020961504 hasLocation W20209615042 @default.
- W2020961504 hasOpenAccess W2020961504 @default.
- W2020961504 hasPrimaryLocation W20209615041 @default.
- W2020961504 hasRelatedWork W1572025105 @default.
- W2020961504 hasRelatedWork W1967446962 @default.
- W2020961504 hasRelatedWork W1971072963 @default.
- W2020961504 hasRelatedWork W2020961504 @default.
- W2020961504 hasRelatedWork W2025654158 @default.
- W2020961504 hasRelatedWork W2040122145 @default.
- W2020961504 hasRelatedWork W2043173001 @default.
- W2020961504 hasRelatedWork W2048440542 @default.
- W2020961504 hasRelatedWork W2127341645 @default.
- W2020961504 hasRelatedWork W2279156773 @default.
- W2020961504 hasVolume "1049" @default.
- W2020961504 isParatext "false" @default.
- W2020961504 isRetracted "false" @default.
- W2020961504 magId "2020961504" @default.
- W2020961504 workType "article" @default.