FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020962162> ?p ?o ?g. }

• W2020962162 endingPage "C456" @default.
• W2020962162 startingPage "C448" @default.
• W2020962162 abstract "Interstitial cells of Cajal (ICC) in the gastrointestinal tract generate and propagate slow waves and mediate neuromuscular neurotransmission. Although damages to ICC have been described in several gastrointestinal motor disorders, analysis of their gene expression in health and disease has been problematic because of the difficulties in isolating these cells. Our goal was to develop techniques for large-scale purification of ICC. Murine ICC were identified in live gastrointestinal muscles with fluorescent Kit antibodies. Because this technique also labels resident macrophages nonspecifically, we attempted to separate ICC from these cells by fluorescence-activated cell sorting with or without immunomagnetic presorting. Efficacy and specificity of ICC purification were tested by quantitative RT-PCR of cell-specific markers. Fluorescence-based separation of small intestinal ICC from unlabeled cells and macrophages tagged with F4/80 antibodies yielded 30,000-40,000 cells and approximately 60-fold enrichment of c-kit mRNA. However, the macrophage marker CD68 was also enriched approximately 6-fold. Magnetic presorting of ICC did not significantly improve selectivity. After labeling contaminating cells with additional paramagnetic (anti-CD11b, -CD11c) and fluorescent antibodies (anti-CD11b) and depleting them by magnetic presorting, we harvested approximately 2,000-4,000 cells from single gastric corpus-antrum muscles and detected an approximately 30-fold increase in c-kit mRNA, no enrichment of mast cells, and an approximately 4-fold reduction of CD68 expression. Adding labeled anti-CD45 antibody to our cocktail further increased c-kit enrichment and eliminated mast cells and macrophages. Smooth muscle cells and myenteric neurons were also depleted. We conclude that immunofluorescence-based sorting can yield ICC in sufficiently high numbers and purity to permit detailed molecular analyses." @default.
• W2020962162 created "2016-06-24" @default.
• W2020962162 creator A5003541727 @default.
• W2020962162 creator A5012332171 @default.
• W2020962162 creator A5021272539 @default.
• W2020962162 creator A5022572500 @default.
• W2020962162 creator A5027145036 @default.
• W2020962162 creator A5028080442 @default.
• W2020962162 creator A5031123252 @default.
• W2020962162 creator A5072411883 @default.
• W2020962162 creator A5091067782 @default.
• W2020962162 date "2004-02-01" @default.
• W2020962162 modified "2023-09-28" @default.
• W2020962162 title "Purification of interstitial cells of Cajal by fluorescence-activated cell sorting" @default.
• W2020962162 cites W1903407475 @default.
• W2020962162 cites W1938170556 @default.
• W2020962162 cites W1968448387 @default.
• W2020962162 cites W1970011482 @default.
• W2020962162 cites W1980429806 @default.
• W2020962162 cites W1981682985 @default.
• W2020962162 cites W1988530903 @default.
• W2020962162 cites W1990194638 @default.
• W2020962162 cites W1991175004 @default.
• W2020962162 cites W1999056716 @default.
• W2020962162 cites W2002590708 @default.
• W2020962162 cites W2005441636 @default.
• W2020962162 cites W2005855950 @default.
• W2020962162 cites W2005900188 @default.
• W2020962162 cites W2014951227 @default.
• W2020962162 cites W2022863975 @default.
• W2020962162 cites W2027636000 @default.
• W2020962162 cites W2031122915 @default.
• W2020962162 cites W2047966069 @default.
• W2020962162 cites W2051669049 @default.
• W2020962162 cites W2052271560 @default.
• W2020962162 cites W2055773218 @default.
• W2020962162 cites W2058400264 @default.
• W2020962162 cites W2058865554 @default.
• W2020962162 cites W2059360956 @default.
• W2020962162 cites W2067405166 @default.
• W2020962162 cites W2100694005 @default.
• W2020962162 cites W2101692831 @default.
• W2020962162 cites W2106451614 @default.
• W2020962162 cites W2114515890 @default.
• W2020962162 cites W2119977924 @default.
• W2020962162 cites W2124058305 @default.
• W2020962162 cites W2127557160 @default.
• W2020962162 cites W2128327026 @default.
• W2020962162 cites W2128681736 @default.
• W2020962162 cites W2150958289 @default.
• W2020962162 cites W2159874981 @default.
• W2020962162 cites W2169478899 @default.
• W2020962162 cites W2253046940 @default.
• W2020962162 cites W2298507042 @default.
• W2020962162 cites W2343287272 @default.
• W2020962162 doi "https://doi.org/10.1152/ajpcell.00273.2003" @default.
• W2020962162 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14534083" @default.
• W2020962162 hasPublicationYear "2004" @default.
• W2020962162 type Work @default.
• W2020962162 sameAs 2020962162 @default.
• W2020962162 citedByCount "31" @default.
• W2020962162 countsByYear W20209621622012 @default.
• W2020962162 countsByYear W20209621622014 @default.
• W2020962162 countsByYear W20209621622015 @default.
• W2020962162 countsByYear W20209621622016 @default.
• W2020962162 countsByYear W20209621622017 @default.
• W2020962162 countsByYear W20209621622018 @default.
• W2020962162 countsByYear W20209621622019 @default.
• W2020962162 countsByYear W20209621622020 @default.
• W2020962162 countsByYear W20209621622021 @default.
• W2020962162 countsByYear W20209621622022 @default.
• W2020962162 countsByYear W20209621622023 @default.
• W2020962162 crossrefType "journal-article" @default.
• W2020962162 hasAuthorship W2020962162A5003541727 @default.
• W2020962162 hasAuthorship W2020962162A5012332171 @default.
• W2020962162 hasAuthorship W2020962162A5021272539 @default.
• W2020962162 hasAuthorship W2020962162A5022572500 @default.
• W2020962162 hasAuthorship W2020962162A5027145036 @default.
• W2020962162 hasAuthorship W2020962162A5028080442 @default.
• W2020962162 hasAuthorship W2020962162A5031123252 @default.
• W2020962162 hasAuthorship W2020962162A5072411883 @default.
• W2020962162 hasAuthorship W2020962162A5091067782 @default.
• W2020962162 hasConcept C104317684 @default.
• W2020962162 hasConcept C127716648 @default.
• W2020962162 hasConcept C142724271 @default.
• W2020962162 hasConcept C153911025 @default.
• W2020962162 hasConcept C159654299 @default.
• W2020962162 hasConcept C185592680 @default.
• W2020962162 hasConcept C201539569 @default.
• W2020962162 hasConcept C203014093 @default.
• W2020962162 hasConcept C204232928 @default.
• W2020962162 hasConcept C2776460901 @default.
• W2020962162 hasConcept C2778630268 @default.
• W2020962162 hasConcept C2779919695 @default.
• W2020962162 hasConcept C2780512811 @default.
• W2020962162 hasConcept C553184892 @default.
• W2020962162 hasConcept C55493867 @default.
• W2020962162 hasConcept C71924100 @default.

• next