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- W2020985124 abstract "Abstract Perchloric acid extracts of tissues were neutralized with tri-N-octylamine and, after removal of ClO4−, subjected to preliminary purification on a Cu2+-loaded column of Chelex 100. A high-pressure liquid chromatographic (HPLC) anion-exchange procedure was developed and gave good resolution of the naturally occurring free nucleotides on a single column. Where heterogeneous peaks eluted, an effective supplementary analysis was achieved by reverse-phase HPLC. An HPLC paired-ion technique was also evaluated for use in nucleotide analysis. Although anion-exchange was best for overall separation of nucleotides, both reverse-phase and paired-ion chromatography gave excellent separation of cyclic nucleotides. Reduced pyridine nucleotides were detected and measured in the form of their acid-decomposition products. The recovery of nucleotides was examined throughout the described analytical techniques and shown to be quantitative." @default.
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- W2020985124 date "1982-07-01" @default.
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- W2020985124 title "Analysis of the free nucleotide pools of mammalian tissues by high-pressure liquid chromatography" @default.
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- W2020985124 doi "https://doi.org/10.1016/0003-2697(82)90461-4" @default.
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