Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021004628> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2021004628 abstract "In the present study, we investigated the role of cAMP-dependent protein kinase in the process of Ca2+ uptake and release from platelet-derived membrane vesicles enriched in the dense tubular system. It was found that these membrane vesicles contain endogenous cAMP-dependent protein kinase and that stimulation of protein kinase by cAMP resulted in the phosphorylation of a single protein band (22 kDa). Addition of cAMP-dependent protein kinase produced effects on vesicle Ca2+ accumulation which were dependent on the Ca2+ concentration in the incubation medium. Specifically, at low extravesicular Ca2+ concentrations, cAMP-dependent protein kinase (10-100 micrograms/ml) produced a dose-dependent stimulation of Ca2+ uptake, however, a similar stimulation was not observed at high extravesicular Ca2+ concentrations. When endogenous protein kinase was blocked by the addition of protein kinase inhibitor, (2-160 nM) there was a dose-dependent inhibition of Ca2+ uptake at both low and high concentrations of extravesicular Ca2+. Furthermore, the addition of protein kinase inhibitor at steady state caused a rapid and dose-dependent release of vesicle-accumulated Ca2+. Studies on the phosphorylation profile of vesicle protein indicated that protein kinase inhibitor (80 and 160 nM) was capable of inhibiting the phosphorylation of the 22-kDa protein within 15 s. Finally, the ability of thromboxane A2 to cause Ca2+ release was inhibited by the addition of cAMP-dependent protein kinase (1 mg/ml). These findings suggest that cAMP-dependent protein kinase is not only a major determinant in the accumulation of Ca2+ by the dense tubular system, but may play an important role in the process of intraplatelet Ca2+ release by physiologic agents such as thromboxane A2." @default.
- W2021004628 created "2016-06-24" @default.
- W2021004628 creator A5049716942 @default.
- W2021004628 creator A5068900915 @default.
- W2021004628 date "1987-10-01" @default.
- W2021004628 modified "2023-10-17" @default.
- W2021004628 title "Modulation of Ca2+ fluxes in isolated platelet vesicles: effects of cAMP-dependent protein kinase and protein kinase inhibitor on Ca2+ sequestration and release" @default.
- W2021004628 cites W1556528051 @default.
- W2021004628 cites W1575000195 @default.
- W2021004628 cites W1583023128 @default.
- W2021004628 cites W1622269784 @default.
- W2021004628 cites W1970038583 @default.
- W2021004628 cites W1983178353 @default.
- W2021004628 cites W1986522994 @default.
- W2021004628 cites W1987385292 @default.
- W2021004628 cites W1993882511 @default.
- W2021004628 cites W2008862271 @default.
- W2021004628 cites W2009811795 @default.
- W2021004628 cites W2014769282 @default.
- W2021004628 cites W2032961622 @default.
- W2021004628 cites W2038853095 @default.
- W2021004628 cites W2039010031 @default.
- W2021004628 cites W2044723736 @default.
- W2021004628 cites W2045988801 @default.
- W2021004628 cites W2050192530 @default.
- W2021004628 cites W2050332669 @default.
- W2021004628 cites W2065993385 @default.
- W2021004628 cites W2068825762 @default.
- W2021004628 cites W2071165662 @default.
- W2021004628 cites W2075130358 @default.
- W2021004628 cites W2086687646 @default.
- W2021004628 cites W2089461338 @default.
- W2021004628 cites W2100837269 @default.
- W2021004628 cites W2117831772 @default.
- W2021004628 cites W2141449078 @default.
- W2021004628 cites W2196351397 @default.
- W2021004628 cites W2284695195 @default.
- W2021004628 cites W2338896395 @default.
- W2021004628 cites W2415572347 @default.
- W2021004628 cites W2610493604 @default.
- W2021004628 doi "https://doi.org/10.1016/0167-4889(87)90049-8" @default.
- W2021004628 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2820511" @default.
- W2021004628 hasPublicationYear "1987" @default.
- W2021004628 type Work @default.
- W2021004628 sameAs 2021004628 @default.
- W2021004628 citedByCount "32" @default.
- W2021004628 countsByYear W20210046282012 @default.
- W2021004628 countsByYear W20210046282015 @default.
- W2021004628 crossrefType "journal-article" @default.
- W2021004628 hasAuthorship W2021004628A5049716942 @default.
- W2021004628 hasAuthorship W2021004628A5068900915 @default.
- W2021004628 hasConcept C159479382 @default.
- W2021004628 hasConcept C184235292 @default.
- W2021004628 hasConcept C185592680 @default.
- W2021004628 hasConcept C195794163 @default.
- W2021004628 hasConcept C199217515 @default.
- W2021004628 hasConcept C2779033064 @default.
- W2021004628 hasConcept C55493867 @default.
- W2021004628 hasConcept C82495950 @default.
- W2021004628 hasConcept C86803240 @default.
- W2021004628 hasConcept C87325107 @default.
- W2021004628 hasConcept C90934575 @default.
- W2021004628 hasConcept C95444343 @default.
- W2021004628 hasConcept C97029542 @default.
- W2021004628 hasConceptScore W2021004628C159479382 @default.
- W2021004628 hasConceptScore W2021004628C184235292 @default.
- W2021004628 hasConceptScore W2021004628C185592680 @default.
- W2021004628 hasConceptScore W2021004628C195794163 @default.
- W2021004628 hasConceptScore W2021004628C199217515 @default.
- W2021004628 hasConceptScore W2021004628C2779033064 @default.
- W2021004628 hasConceptScore W2021004628C55493867 @default.
- W2021004628 hasConceptScore W2021004628C82495950 @default.
- W2021004628 hasConceptScore W2021004628C86803240 @default.
- W2021004628 hasConceptScore W2021004628C87325107 @default.
- W2021004628 hasConceptScore W2021004628C90934575 @default.
- W2021004628 hasConceptScore W2021004628C95444343 @default.
- W2021004628 hasConceptScore W2021004628C97029542 @default.
- W2021004628 hasLocation W20210046281 @default.
- W2021004628 hasLocation W20210046282 @default.
- W2021004628 hasOpenAccess W2021004628 @default.
- W2021004628 hasPrimaryLocation W20210046281 @default.
- W2021004628 hasRelatedWork W131448777 @default.
- W2021004628 hasRelatedWork W1507353711 @default.
- W2021004628 hasRelatedWork W1610924477 @default.
- W2021004628 hasRelatedWork W1985386404 @default.
- W2021004628 hasRelatedWork W2035096705 @default.
- W2021004628 hasRelatedWork W2036777733 @default.
- W2021004628 hasRelatedWork W2038206601 @default.
- W2021004628 hasRelatedWork W2044532513 @default.
- W2021004628 hasRelatedWork W2073581382 @default.
- W2021004628 hasRelatedWork W2396104867 @default.
- W2021004628 isParatext "false" @default.
- W2021004628 isRetracted "false" @default.
- W2021004628 magId "2021004628" @default.
- W2021004628 workType "article" @default.