FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021005426> ?p ?o ?g. }

• W2021005426 endingPage "215" @default.
• W2021005426 startingPage "209" @default.
• W2021005426 abstract "Little is known about the structure of prolactin (PRL) within secretory granules. Evidence from our previous studies in bovine tissue preparations suggests that control of secretion may reside, in part, in the conversion of storage hormone to releasable PRL. The conversion can be monitored by measuring changes in immunodetectability since the oligomeric, storage form is poorly recognized by antisera raised against monomeric PRL. Since many investigators use rats to study the secretory process and changes in detectability of rat pituitary PRL occur during lactation (“depletion-transformation”), we undertook the present immunodetectability studies to gain insight into the storage structure of rat (r) PRL. Cysteamine and zinc inhibited tissue PRL immunoassayability in male rat pituitary homogenates and also in partially purified secretory granules as they had inhibited bovine (b) PRL; however, zinc inhibited the rodent hormone less potently than the bovine. In vitro incubation of rat tissue samples without additions resulted in increases in rPRL detectability of up to 84% after 180 minutes; such incubation of bovine samples had no significant effect. A striking additional difference between the species was that exposure to reduced glutathione (GSH), cysteine, homocysteine, mercaptoethanol, and dithiothreitol inhibited rPRL by up to 44%. This compared to thiol stimulation of bPRL by as much as 450%. The inhibitory GSH effect on rPRL was abolished when 0.5% sodium dodecyl sulfate (SDS) was included; in contrast, the stimulatory GSH effect on bPRL did not change with added SDS. SDS alone had no effect on rat homogenate PRL, and only increased rat granule rPRL by 23% compared to its ability to increase bPRL assayability by 44%. High performance liquid chromatography demonstrated a high molecular weight peak of immunoreactive rat PRL. This was largely abolished, with a simultaneous increase in monomer and intermediate zone material, by treatment of extracts with either GSH or 4 mol/L urea. The immunoreactivity of the high molecular weight peak was increased nearly 50% by treatment with GSH. The striking qualitative discrepancy between rPRL and bPRL responses to GSH and other thiols cannot be accounted for by dosage of exposure, the use of fresh v frozen tissue, or old v young donor animals. The mode of hormone packaging within secretory granules or differences in nonhormonal granule components (eg, enzymes) may explain the differential responses. Alternatively, since only a small amount of the total rPRL is oligomeric, the differences may be partly explained by major GSH action on intramolecular rPRL bonds, with a tendency to decrease monomer immunoactivity, v major GSH action on intermolecular bonds in bPRL, with generation of monomers. These results indicate that if thiol:disulfide mechanisms are an important feature of rPRL as well as of bPRL tissue storage, the details must differ between the species." @default.
• W2021005426 created "2016-06-24" @default.
• W2021005426 creator A5055394431 @default.
• W2021005426 creator A5068889024 @default.
• W2021005426 date "1986-03-01" @default.
• W2021005426 modified "2023-09-30" @default.
• W2021005426 title "Cysteamine, zinc, and thiols modify detectability of rat pituitary prolactin: A comparison with effects on bovine prolactin suggests differences in hormone storage" @default.
• W2021005426 cites W1188871022 @default.
• W2021005426 cites W1479999619 @default.
• W2021005426 cites W1527932552 @default.
• W2021005426 cites W1582951558 @default.
• W2021005426 cites W1775749144 @default.
• W2021005426 cites W190883241 @default.
• W2021005426 cites W1980245435 @default.
• W2021005426 cites W1991622522 @default.
• W2021005426 cites W2016266254 @default.
• W2021005426 cites W2020658922 @default.
• W2021005426 cites W2028340765 @default.
• W2021005426 cites W2037898551 @default.
• W2021005426 cites W2066875273 @default.
• W2021005426 cites W2069920773 @default.
• W2021005426 cites W2071539853 @default.
• W2021005426 cites W2082785616 @default.
• W2021005426 cites W2092244418 @default.
• W2021005426 cites W2111708824 @default.
• W2021005426 cites W2116918369 @default.
• W2021005426 cites W2130982713 @default.
• W2021005426 cites W2131990717 @default.
• W2021005426 cites W2142057904 @default.
• W2021005426 cites W2203399240 @default.
• W2021005426 cites W241725786 @default.
• W2021005426 cites W4245957869 @default.
• W2021005426 doi "https://doi.org/10.1016/0026-0495(86)90203-9" @default.
• W2021005426 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3081777" @default.
• W2021005426 hasPublicationYear "1986" @default.
• W2021005426 type Work @default.
• W2021005426 sameAs 2021005426 @default.
• W2021005426 citedByCount "10" @default.
• W2021005426 countsByYear W20210054262019 @default.
• W2021005426 countsByYear W20210054262021 @default.
• W2021005426 crossrefType "journal-article" @default.
• W2021005426 hasAuthorship W2021005426A5055394431 @default.
• W2021005426 hasAuthorship W2021005426A5068889024 @default.
• W2021005426 hasConcept C126322002 @default.
• W2021005426 hasConcept C134018914 @default.
• W2021005426 hasConcept C181199279 @default.
• W2021005426 hasConcept C185592680 @default.
• W2021005426 hasConcept C25642318 @default.
• W2021005426 hasConcept C2776609658 @default.
• W2021005426 hasConcept C2776659692 @default.
• W2021005426 hasConcept C2777658017 @default.
• W2021005426 hasConcept C2779029589 @default.
• W2021005426 hasConcept C2779064019 @default.
• W2021005426 hasConcept C2779201268 @default.
• W2021005426 hasConcept C2779234561 @default.
• W2021005426 hasConcept C538909803 @default.
• W2021005426 hasConcept C54355233 @default.
• W2021005426 hasConcept C55493867 @default.
• W2021005426 hasConcept C71315377 @default.
• W2021005426 hasConcept C71924100 @default.
• W2021005426 hasConcept C86803240 @default.
• W2021005426 hasConceptScore W2021005426C126322002 @default.
• W2021005426 hasConceptScore W2021005426C134018914 @default.
• W2021005426 hasConceptScore W2021005426C181199279 @default.
• W2021005426 hasConceptScore W2021005426C185592680 @default.
• W2021005426 hasConceptScore W2021005426C25642318 @default.
• W2021005426 hasConceptScore W2021005426C2776609658 @default.
• W2021005426 hasConceptScore W2021005426C2776659692 @default.
• W2021005426 hasConceptScore W2021005426C2777658017 @default.
• W2021005426 hasConceptScore W2021005426C2779029589 @default.
• W2021005426 hasConceptScore W2021005426C2779064019 @default.
• W2021005426 hasConceptScore W2021005426C2779201268 @default.
• W2021005426 hasConceptScore W2021005426C2779234561 @default.
• W2021005426 hasConceptScore W2021005426C538909803 @default.
• W2021005426 hasConceptScore W2021005426C54355233 @default.
• W2021005426 hasConceptScore W2021005426C55493867 @default.
• W2021005426 hasConceptScore W2021005426C71315377 @default.
• W2021005426 hasConceptScore W2021005426C71924100 @default.
• W2021005426 hasConceptScore W2021005426C86803240 @default.
• W2021005426 hasIssue "3" @default.
• W2021005426 hasLocation W20210054261 @default.
• W2021005426 hasLocation W20210054262 @default.
• W2021005426 hasOpenAccess W2021005426 @default.
• W2021005426 hasPrimaryLocation W20210054261 @default.
• W2021005426 hasRelatedWork W1966296538 @default.
• W2021005426 hasRelatedWork W1971415547 @default.
• W2021005426 hasRelatedWork W2000124881 @default.
• W2021005426 hasRelatedWork W2013119480 @default.
• W2021005426 hasRelatedWork W2032920953 @default.
• W2021005426 hasRelatedWork W2050420293 @default.
• W2021005426 hasRelatedWork W2056934175 @default.
• W2021005426 hasRelatedWork W2069818517 @default.
• W2021005426 hasRelatedWork W2154189055 @default.
• W2021005426 hasRelatedWork W2167331895 @default.
• W2021005426 hasVolume "35" @default.
• W2021005426 isParatext "false" @default.
• W2021005426 isRetracted "false" @default.
• W2021005426 magId "2021005426" @default.

• next