FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021011803> ?p ?o ?g. }

• W2021011803 endingPage "11323" @default.
• W2021011803 startingPage "11312" @default.
• W2021011803 abstract "Coronaviruses are the enveloped, positive-stranded RNA viruses with the largest RNA genomes known. Several features make these viruses attractive as vaccine and therapeutic vectors: (i) deletion of their nonessential genes is strongly attenuating; (ii) the genetic space thus created allows insertion of foreign information; and (iii) their tropism can be modified by manipulation of the viral spike. We studied here their ability to serve as expression vectors by inserting two different foreign genes and evaluating systematically the genomic position dependence of their expression, using a murine coronavirus as a model. Renilla and firefly luciferase expression cassettes, each provided with viral transcription regulatory sequences (TRSs), were inserted at several genomic positions, both independently in different viruses and combined within one viral genome. Recombinant viruses were generated by using a convenient method based on targeted recombination and host cell switching. In all cases high expression levels of the foreign genes were observed without severe effects on viral replication in vitro. The expression of the inserted gene appeared to be dependent on its genomic position, as well as on the identity of the gene. Expression levels increased when the luciferase gene was inserted closer to the 3' end of the genome. The foreign gene insertions generally reduced the expression of upstream viral genes. The results are consistent with coronavirus transcription models in which the transcription from upstream TRSs is attenuated by downstream TRSs. Altogether, our observations clearly demonstrate the potential of coronaviruses as (multivalent) expression vectors." @default.
• W2021011803 created "2016-06-24" @default.
• W2021011803 creator A5000266713 @default.
• W2021011803 creator A5020489800 @default.
• W2021011803 creator A5028105148 @default.
• W2021011803 creator A5032673596 @default.
• W2021011803 creator A5080414643 @default.
• W2021011803 date "2003-11-01" @default.
• W2021011803 modified "2023-09-25" @default.
• W2021011803 title "Coronaviruses as Vectors: Position Dependence of Foreign Gene Expression" @default.
• W2021011803 cites W1582853114 @default.
• W2021011803 cites W1608425304 @default.
• W2021011803 cites W1626663091 @default.
• W2021011803 cites W1811783050 @default.
• W2021011803 cites W1912791812 @default.
• W2021011803 cites W1933968634 @default.
• W2021011803 cites W1952254443 @default.
• W2021011803 cites W1962505022 @default.
• W2021011803 cites W1965956757 @default.
• W2021011803 cites W1968038125 @default.
• W2021011803 cites W1989191197 @default.
• W2021011803 cites W1994955540 @default.
• W2021011803 cites W1995301907 @default.
• W2021011803 cites W1996259826 @default.
• W2021011803 cites W2012605222 @default.
• W2021011803 cites W2012735174 @default.
• W2021011803 cites W2036093415 @default.
• W2021011803 cites W2040734574 @default.
• W2021011803 cites W2053027085 @default.
• W2021011803 cites W2056636829 @default.
• W2021011803 cites W2066715749 @default.
• W2021011803 cites W2067981792 @default.
• W2021011803 cites W2086888574 @default.
• W2021011803 cites W2099450221 @default.
• W2021011803 cites W2103019311 @default.
• W2021011803 cites W2103050864 @default.
• W2021011803 cites W2105111946 @default.
• W2021011803 cites W2110201489 @default.
• W2021011803 cites W2123765122 @default.
• W2021011803 cites W2126980050 @default.
• W2021011803 cites W2127226387 @default.
• W2021011803 cites W2131344386 @default.
• W2021011803 cites W2131662589 @default.
• W2021011803 cites W2131887043 @default.
• W2021011803 cites W2132592630 @default.
• W2021011803 cites W2133256064 @default.
• W2021011803 cites W2135629385 @default.
• W2021011803 cites W2136118315 @default.
• W2021011803 cites W2136145315 @default.
• W2021011803 cites W2140790746 @default.
• W2021011803 cites W2142020584 @default.
• W2021011803 cites W2143257062 @default.
• W2021011803 cites W2145657635 @default.
• W2021011803 cites W2148679505 @default.
• W2021011803 cites W2154763448 @default.
• W2021011803 cites W2163954393 @default.
• W2021011803 cites W2166185221 @default.
• W2021011803 cites W3011200155 @default.
• W2021011803 cites W72835126 @default.
• W2021011803 cites W97600987 @default.
• W2021011803 doi "https://doi.org/10.1128/jvi.77.21.11312-11323.2003" @default.
• W2021011803 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/229330" @default.
• W2021011803 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14557617" @default.
• W2021011803 hasPublicationYear "2003" @default.
• W2021011803 type Work @default.
• W2021011803 sameAs 2021011803 @default.
• W2021011803 citedByCount "69" @default.
• W2021011803 countsByYear W20210118032012 @default.
• W2021011803 countsByYear W20210118032013 @default.
• W2021011803 countsByYear W20210118032014 @default.
• W2021011803 countsByYear W20210118032015 @default.
• W2021011803 countsByYear W20210118032016 @default.
• W2021011803 countsByYear W20210118032017 @default.
• W2021011803 countsByYear W20210118032018 @default.
• W2021011803 countsByYear W20210118032019 @default.
• W2021011803 countsByYear W20210118032020 @default.
• W2021011803 countsByYear W20210118032021 @default.
• W2021011803 countsByYear W20210118032022 @default.
• W2021011803 crossrefType "journal-article" @default.
• W2021011803 hasAuthorship W2021011803A5000266713 @default.
• W2021011803 hasAuthorship W2021011803A5020489800 @default.
• W2021011803 hasAuthorship W2021011803A5028105148 @default.
• W2021011803 hasAuthorship W2021011803A5032673596 @default.
• W2021011803 hasAuthorship W2021011803A5080414643 @default.
• W2021011803 hasBestOaLocation W20210118032 @default.
• W2021011803 hasConcept C104317684 @default.
• W2021011803 hasConcept C138885662 @default.
• W2021011803 hasConcept C141231307 @default.
• W2021011803 hasConcept C142724271 @default.
• W2021011803 hasConcept C150194340 @default.
• W2021011803 hasConcept C159047783 @default.
• W2021011803 hasConcept C179926584 @default.
• W2021011803 hasConcept C2777648638 @default.
• W2021011803 hasConcept C2779134260 @default.
• W2021011803 hasConcept C3008058167 @default.
• W2021011803 hasConcept C41895202 @default.
• W2021011803 hasConcept C524204448 @default.
• W2021011803 hasConcept C54355233 @default.

• next