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- W2021024438 abstract "Somatostatin has been reported to decrease with age in many brain regions and these changes generally have been considered to have important implications for the regulation of both neural activity and neuroendocrine regulatory systems. The purpose of this study was to determine whether the age-related changes in somatostatin concentration in cortex and hypothalamus are attributable to alterations in the regulation of somatostatin gene expression. Hypothalamic and cortical tissue were dissected from young (3–4 month), middle-aged (12–14 month), and old (22 month) male Fischer 344 rats. Total RNA was extracted and dilutions blotted to nitrocellulose. Somatostatin cDNA in expression vector pSP65 was used to produce a 32P-labeled antisense probe for hybridization. After washing, blots were autoradiographed and analyzed by densitometry. Dilutions of total RNA were also probed with 32P-labeled oligo d(pT)16 to determine poly A+RNA levels. Data were expressed as relative somatostatin gene expression (somatostatin mRNA/poly A+RNA). Results indicated that in cortex, relative somatostatin gene expression was similar in young, middle-aged, and old animals (0.54 ± 0.11, 0.60 ± 0.08, and 0.51 ± 0.04, respectively). However, somatostatin gene expression in the hypothalamus decreased consistently with age and ratios in old rats were approximately 50% of levels observed in young animals (p < 0.05). Northern analysis of RNA revealed a single somatostatin transcript of approximately 0.65 kb in all age groups. In situ hybridization analysis of somatostatin mRNA in the hypothalamus indicated that the age-related decrease in somatostatin gene expression is a consequence of decreased expression within specific hypothalamic nuclei rather than a loss of somatostatin-containing neurons. Our results indicate that 1) somatostatin gene expression decreases in hypothalamus but not in cortex of aging rats, 2) the decrease in hypothalamic somatostatin mRNA is due to a decrease in gene expression per cell, and 3) there are no apparent changes in the size of the somatostatin transcript with age. We conclude that increases in steady-state levels of somatostatin mRNA are not a causative factor in the reduction in growth hormone with age. Therefore, relative somatostatin/GRF gene expression or alterations in somatostatin posttranslational processes may be responsible for the decline in growth hormone and these changes may be an early consequence of brain aging." @default.
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- W2021024438 date "1990-07-01" @default.
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- W2021024438 title "Somatostatin gene expression in hypothalamus and cortex of aging male rats" @default.
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- W2021024438 doi "https://doi.org/10.1016/0197-4580(90)90007-m" @default.
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