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- W2021035708 abstract "We have developed a simple, rapid and relatively inexpensive protocol for direct non-isotopic cycle sequencing of DNA amplified using the polymerase chain reaction (PCR). PCR is performed on routine and archival neuropathological tissue. For sequencing, a 5'-digoxigenin end-labelled oligonucleotide primer is annealed and extended during thermal cycling, sequencing reactions are separated on a standard sequencing gel and the gel is contact-blotted to a nylon membrane. Sequenced DNA is visualized using immunological detection of digoxigenin." @default.
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- W2021035708 date "1993-10-01" @default.
- W2021035708 modified "2023-10-14" @default.
- W2021035708 title "Non-Radioactive Direct Sequencing of PCR Products Amplified from Neuropathological Specimens" @default.
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- W2021035708 doi "https://doi.org/10.1111/j.1750-3639.1993.tb00770.x" @default.
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