FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021051549> ?p ?o ?g. }

• W2021051549 endingPage "106" @default.
• W2021051549 startingPage "97" @default.
• W2021051549 abstract "Inflammation, characterized by the presence of activated microglia and reactive astrocytes (gliosis), has been described in Alzheimer's disease (AD). We used our routine single immunohistochemical (IHC) labeling protocol to label amyloid plaques, an AD neuropathological hallmark, activated microglia, and reactive astrocytes in serial sections of AD hippocampus and entorhinal cortex of brain. Although most amyloid plaques were associated with inflammation throughout the serial sections, the extent of microglial and astrocytic activation varied among the amyloid plaques. We also observed a population of amyloid plaques that did not appear to coincide with immunolabeled microglia and astrocytes in serial sections, leading us to speculate that some amyloid plaques are not associated with inflammation. Because serial sectioning limited our ability to confirm these findings, we developed a triple IHC protocol to investigate the association of activated microglia and reactive astrocytes simultaneously with amyloid plaques in sections of AD brain entorhinal cortex and hippocampus. Unlike the potential errors of extrapolating descriptive information from routine IHC or histochemical staining methods on sectioned tissues, triple IHC allowed direct characterization of three differently colored antigens in situ. The success of the protocol depended on selection of distinguishable color schemes and resolution of other critical technical elements including the compatibility of the reagents and the sensitivity and sequence of the detection systems. The results of the triple IHC protocol clarified the spatial relation of microglia and astrocytes with amyloid plaques and provoked novel interpretations about the roles of inflammation in AD brain tissues. We categorized three distinct populations of amyloid plaques related to of inflammation: 1) Abeta42 immunoreactive (a marker of amyloid plaques) amyloid plaques without activated microglia or reactive astrocytes, 2) Abeta42-positive amyloid plaques with HLA-DR (a marker of microglia)-positive microglia and no astrocytes, 3) Abeta42-positive amyloid plaques among HLA-DR and GFAP (a marker of astrocytes) immunoreactive astrocytes. Most amyloid plaques had varying degrees of activated microglia and reactive astrocytes. Some of the amyloid plaques were not associated with inflammation while others were associated only with activated microglia. These findings suggest that amyloid plaques without associated inflammation may represent recently formed plaques and that the presence of amyloid plaques in AD brains may activate microglia prior to gliosis. Furthermore, the shape of the amyloid plaques may be altered subsequently from its typical spherical to an aspherical shape by the inflammatory cells." @default.
• W2021051549 created "2016-06-24" @default.
• W2021051549 creator A5004990133 @default.
• W2021051549 creator A5025169559 @default.
• W2021051549 creator A5038622138 @default.
• W2021051549 creator A5041254259 @default.
• W2021051549 creator A5042045393 @default.
• W2021051549 date "2001-01-01" @default.
• W2021051549 modified "2023-10-17" @default.
• W2021051549 title "Application of triple immunohistochemistry to characterize amyloid plaque-associated inflammation in brains with Alzheimer's disease" @default.
• W2021051549 cites W2036288989 @default.
• W2021051549 cites W2142437726 @default.
• W2021051549 cites W2164956116 @default.
• W2021051549 doi "https://doi.org/10.1080/bih.76.2.97.106" @default.
• W2021051549 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11440311" @default.
• W2021051549 hasPublicationYear "2001" @default.
• W2021051549 type Work @default.
• W2021051549 sameAs 2021051549 @default.
• W2021051549 citedByCount "49" @default.
• W2021051549 countsByYear W20210515492012 @default.
• W2021051549 countsByYear W20210515492013 @default.
• W2021051549 countsByYear W20210515492014 @default.
• W2021051549 countsByYear W20210515492015 @default.
• W2021051549 countsByYear W20210515492016 @default.
• W2021051549 countsByYear W20210515492017 @default.
• W2021051549 countsByYear W20210515492018 @default.
• W2021051549 countsByYear W20210515492019 @default.
• W2021051549 countsByYear W20210515492020 @default.
• W2021051549 countsByYear W20210515492021 @default.
• W2021051549 countsByYear W20210515492022 @default.
• W2021051549 countsByYear W20210515492023 @default.
• W2021051549 crossrefType "journal-article" @default.
• W2021051549 hasAuthorship W2021051549A5004990133 @default.
• W2021051549 hasAuthorship W2021051549A5025169559 @default.
• W2021051549 hasAuthorship W2021051549A5038622138 @default.
• W2021051549 hasAuthorship W2021051549A5041254259 @default.
• W2021051549 hasAuthorship W2021051549A5042045393 @default.
• W2021051549 hasConcept C142724271 @default.
• W2021051549 hasConcept C169760540 @default.
• W2021051549 hasConcept C203014093 @default.
• W2021051549 hasConcept C204232928 @default.
• W2021051549 hasConcept C2776914184 @default.
• W2021051549 hasConcept C2777633098 @default.
• W2021051549 hasConcept C2777682930 @default.
• W2021051549 hasConcept C2779134260 @default.
• W2021051549 hasConcept C2779830541 @default.
• W2021051549 hasConcept C2780715579 @default.
• W2021051549 hasConcept C2781161787 @default.
• W2021051549 hasConcept C2993858690 @default.
• W2021051549 hasConcept C502032728 @default.
• W2021051549 hasConcept C68026918 @default.
• W2021051549 hasConcept C71924100 @default.
• W2021051549 hasConcept C86803240 @default.
• W2021051549 hasConceptScore W2021051549C142724271 @default.
• W2021051549 hasConceptScore W2021051549C169760540 @default.
• W2021051549 hasConceptScore W2021051549C203014093 @default.
• W2021051549 hasConceptScore W2021051549C204232928 @default.
• W2021051549 hasConceptScore W2021051549C2776914184 @default.
• W2021051549 hasConceptScore W2021051549C2777633098 @default.
• W2021051549 hasConceptScore W2021051549C2777682930 @default.
• W2021051549 hasConceptScore W2021051549C2779134260 @default.
• W2021051549 hasConceptScore W2021051549C2779830541 @default.
• W2021051549 hasConceptScore W2021051549C2780715579 @default.
• W2021051549 hasConceptScore W2021051549C2781161787 @default.
• W2021051549 hasConceptScore W2021051549C2993858690 @default.
• W2021051549 hasConceptScore W2021051549C502032728 @default.
• W2021051549 hasConceptScore W2021051549C68026918 @default.
• W2021051549 hasConceptScore W2021051549C71924100 @default.
• W2021051549 hasConceptScore W2021051549C86803240 @default.
• W2021051549 hasIssue "2" @default.
• W2021051549 hasLocation W20210515491 @default.
• W2021051549 hasLocation W20210515492 @default.
• W2021051549 hasOpenAccess W2021051549 @default.
• W2021051549 hasPrimaryLocation W20210515491 @default.
• W2021051549 hasRelatedWork W1515550820 @default.
• W2021051549 hasRelatedWork W1966222819 @default.
• W2021051549 hasRelatedWork W1973238388 @default.
• W2021051549 hasRelatedWork W2021051549 @default.
• W2021051549 hasRelatedWork W2046725122 @default.
• W2021051549 hasRelatedWork W2098836351 @default.
• W2021051549 hasRelatedWork W2106003388 @default.
• W2021051549 hasRelatedWork W2280733710 @default.
• W2021051549 hasRelatedWork W2461349049 @default.
• W2021051549 hasRelatedWork W4237363447 @default.
• W2021051549 hasVolume "76" @default.
• W2021051549 isParatext "false" @default.
• W2021051549 isRetracted "false" @default.
• W2021051549 magId "2021051549" @default.
• W2021051549 workType "article" @default.