FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021051603> ?p ?o ?g. }

• W2021051603 endingPage "183" @default.
• W2021051603 startingPage "177" @default.
• W2021051603 abstract "DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 40:177-183 (2000) - doi:10.3354/dao040177 Multiplex PCR assay for ureC and 16S rRNA genes clearly discriminates between both subspecies of Photobacterium damselae C. R. Osorio*, A. E. Toranzo, J. L. Romalde, J. L. Barja Departamento de Microbiología y Parasitología, Facultad de Biología and Instituto de Acuicultura, Universidad de Santiago, 15706, Santiago de Compostela, Spain *E-mail: mpaetjlb@usc.es ABSTRACT: A multiplex-PCR approach, employing 2 primer pairs directed to internal regions of the 16S rRNA and ureC genes, was utilized to analyze a collection of Photobacterium damselae strains, including 25 isolates of subspecies piscicida and 15 isolates of subspecies damselae. With this procedure, all the P. damselae subsp. damselae strains yielded 2 amplification products, one of 267 bp and the other of 448 bp, corresponding to internal fragments of the 16S rRNA and ureC genes, respectively. However, P. damselae subsp. piscicida isolates only showed the PCR product of 267 bp (16S rRNA fragment), indicating the absence of the urease gene in its genome. We have constructed a DNA probe directed to an internal region of the ureC gene, and corroborated by dot blot hybridization that the P. damselae subsp. piscicida lacks this gene, whereas it is present in the subspecies damselae. This constitutes the first successful discrimination between both subspecies using a PCR procedure, which could become a useful tool for diagnosis of pasteurellosis in the field. In addition, since these 2 subspecies have been shown to share nearly the same rrn operon sequence, our results provided evidence that one of the steps in the P. damselae speciation proccess included gain/loss events associated with the ure operon. KEY WORDS: Multiplex-PCR · Photobacterium damselae · ureC gene · 16S rRNA gene · Subspecies discrimination Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 40, No. 3. Publication date: April 20, 2000 Print ISSN:0177-5103; Online ISSN:1616-1580 Copyright © 2000 Inter-Research." @default.
• W2021051603 created "2016-06-24" @default.
• W2021051603 creator A5003743787 @default.
• W2021051603 creator A5048792453 @default.
• W2021051603 creator A5073988281 @default.
• W2021051603 creator A5088137068 @default.
• W2021051603 date "2000-01-01" @default.
• W2021051603 modified "2023-09-25" @default.
• W2021051603 title "Multiplex PCR assay for ureC and 16S rRNA genes clearly discriminates between both subspecies of Photobacterium damselae" @default.
• W2021051603 cites W2027318497 @default.
• W2021051603 doi "https://doi.org/10.3354/dao040177" @default.
• W2021051603 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10843555" @default.
• W2021051603 hasPublicationYear "2000" @default.
• W2021051603 type Work @default.
• W2021051603 sameAs 2021051603 @default.
• W2021051603 citedByCount "82" @default.
• W2021051603 countsByYear W20210516032012 @default.
• W2021051603 countsByYear W20210516032013 @default.
• W2021051603 countsByYear W20210516032014 @default.
• W2021051603 countsByYear W20210516032015 @default.
• W2021051603 countsByYear W20210516032016 @default.
• W2021051603 countsByYear W20210516032017 @default.
• W2021051603 countsByYear W20210516032018 @default.
• W2021051603 countsByYear W20210516032019 @default.
• W2021051603 countsByYear W20210516032020 @default.
• W2021051603 countsByYear W20210516032021 @default.
• W2021051603 countsByYear W20210516032022 @default.
• W2021051603 countsByYear W20210516032023 @default.
• W2021051603 crossrefType "journal-article" @default.
• W2021051603 hasAuthorship W2021051603A5003743787 @default.
• W2021051603 hasAuthorship W2021051603A5048792453 @default.
• W2021051603 hasAuthorship W2021051603A5073988281 @default.
• W2021051603 hasAuthorship W2021051603A5088137068 @default.
• W2021051603 hasBestOaLocation W20210516031 @default.
• W2021051603 hasConcept C104317684 @default.
• W2021051603 hasConcept C17966761 @default.
• W2021051603 hasConcept C2776154503 @default.
• W2021051603 hasConcept C2780086907 @default.
• W2021051603 hasConcept C42062724 @default.
• W2021051603 hasConcept C49105822 @default.
• W2021051603 hasConcept C523546767 @default.
• W2021051603 hasConcept C54355233 @default.
• W2021051603 hasConcept C67905577 @default.
• W2021051603 hasConcept C86803240 @default.
• W2021051603 hasConcept C89423630 @default.
• W2021051603 hasConcept C90583042 @default.
• W2021051603 hasConcept C90856448 @default.
• W2021051603 hasConceptScore W2021051603C104317684 @default.
• W2021051603 hasConceptScore W2021051603C17966761 @default.
• W2021051603 hasConceptScore W2021051603C2776154503 @default.
• W2021051603 hasConceptScore W2021051603C2780086907 @default.
• W2021051603 hasConceptScore W2021051603C42062724 @default.
• W2021051603 hasConceptScore W2021051603C49105822 @default.
• W2021051603 hasConceptScore W2021051603C523546767 @default.
• W2021051603 hasConceptScore W2021051603C54355233 @default.
• W2021051603 hasConceptScore W2021051603C67905577 @default.
• W2021051603 hasConceptScore W2021051603C86803240 @default.
• W2021051603 hasConceptScore W2021051603C89423630 @default.
• W2021051603 hasConceptScore W2021051603C90583042 @default.
• W2021051603 hasConceptScore W2021051603C90856448 @default.
• W2021051603 hasLocation W20210516031 @default.
• W2021051603 hasLocation W20210516032 @default.
• W2021051603 hasOpenAccess W2021051603 @default.
• W2021051603 hasPrimaryLocation W20210516031 @default.
• W2021051603 hasRelatedWork W1636029445 @default.
• W2021051603 hasRelatedWork W1971228535 @default.
• W2021051603 hasRelatedWork W1973422339 @default.
• W2021051603 hasRelatedWork W2028790803 @default.
• W2021051603 hasRelatedWork W2031310766 @default.
• W2021051603 hasRelatedWork W2131339900 @default.
• W2021051603 hasRelatedWork W2380586364 @default.
• W2021051603 hasRelatedWork W2384777167 @default.
• W2021051603 hasRelatedWork W2408310872 @default.
• W2021051603 hasRelatedWork W3030711601 @default.
• W2021051603 hasVolume "40" @default.
• W2021051603 isParatext "false" @default.
• W2021051603 isRetracted "false" @default.
• W2021051603 magId "2021051603" @default.
• W2021051603 workType "article" @default.