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• W2021052544 startingPage "1244" @default.
• W2021052544 abstract "Kallikrein-related peptidases (KLKs) are a family of serine proteases that have been shown to be dysregulated in several malignancies including ovarian cancer. The control of kallikrein genes and their physiological function in cancer is not well understood. We hypothesized that microRNAs (miRNAs) represent a novel mechanism for post-transcriptional control of KLK expression in cancer. We first analysed miRNA expression in ovarian cancer in silico. A total of 98 miRNAs were reported to have altered expression in ovarian cancer. Three of these miRNAs were predicted to target KLK10. We experimentally verified the predicted miR–KLK10 interaction using two independent techniques, a luciferase assay with a construct containing the KLK10 3′ untranslated region (UTR), pMIR–KLK10, and measuring KLK10 protein levels after transfection with miRNA. When we co-transfected cells with pMIR–KLK10 and either let-7f, miR-224, or mR-516a, we saw decreased luciferase signal, suggesting that these miRNAs can target KLK10. We then examined the effect of these three miRNAs on KLK10 protein expression and cell growth. Transfection of all miRNAs, let-7f, miR-224, and miR-516a led to a decrease in protein expression and cellular growth. This effect was shown to be dose dependent. The KLK10 protein levels were partially restored by co-transfecting let-7f and its inhibitor. In addition, there was a slight decrease in KLK10 mRNA expression after transfection with let-7f. Our results confirm that KLKs can be targeted by more than one miRNA. Increased expression of certain miRNAs in ovarian cancer can lead to decreased KLK protein expression and subsequently have a negative effect on cell proliferation. This dose-dependent effect suggests that a ‘tweaking’ or ‘fine-tuning’ mechanism exists in which the expression of one KLK can be controlled by multiple miRNAs. These data together suggest that miRNA may be used as potential therapeutic options and further studies are required." @default.
• W2021052544 created "2016-06-24" @default.
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• W2021052544 date "2010-03-30" @default.
• W2021052544 modified "2023-10-06" @default.
• W2021052544 title "Three dysregulated miRNAs control kallikrein 10 expression and cell proliferation in ovarian cancer" @default.
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• W2021052544 cites W1966503229 @default.
• W2021052544 cites W1968313287 @default.
• W2021052544 cites W1973220599 @default.
• W2021052544 cites W1978337436 @default.
• W2021052544 cites W1984754167 @default.
• W2021052544 cites W1985402095 @default.
• W2021052544 cites W1997870903 @default.
• W2021052544 cites W1999473606 @default.
• W2021052544 cites W2002995168 @default.
• W2021052544 cites W2006122379 @default.
• W2021052544 cites W2010183271 @default.
• W2021052544 cites W2019155015 @default.
• W2021052544 cites W2023858596 @default.
• W2021052544 cites W2024066414 @default.
• W2021052544 cites W2026968612 @default.
• W2021052544 cites W2035844407 @default.
• W2021052544 cites W2036224487 @default.
• W2021052544 cites W2038696333 @default.
• W2021052544 cites W2039223093 @default.
• W2021052544 cites W2040191060 @default.
• W2021052544 cites W2041421154 @default.
• W2021052544 cites W2051156280 @default.
• W2021052544 cites W2053490054 @default.
• W2021052544 cites W2058059940 @default.
• W2021052544 cites W2062925537 @default.
• W2021052544 cites W2063333517 @default.
• W2021052544 cites W2065109700 @default.
• W2021052544 cites W2065899119 @default.
• W2021052544 cites W2066442082 @default.
• W2021052544 cites W2067135009 @default.
• W2021052544 cites W2076841583 @default.
• W2021052544 cites W2080832370 @default.
• W2021052544 cites W2083381199 @default.
• W2021052544 cites W2088738576 @default.
• W2021052544 cites W2089977713 @default.
• W2021052544 cites W2090219560 @default.
• W2021052544 cites W2091651379 @default.
• W2021052544 cites W2096382704 @default.
• W2021052544 cites W2096573862 @default.
• W2021052544 cites W2096731051 @default.
• W2021052544 cites W2097257058 @default.
• W2021052544 cites W2100048800 @default.
• W2021052544 cites W2102948567 @default.
• W2021052544 cites W2109602297 @default.
• W2021052544 cites W2109834412 @default.
• W2021052544 cites W2110440423 @default.
• W2021052544 cites W2112720380 @default.
• W2021052544 cites W2112740613 @default.
• W2021052544 cites W2117461801 @default.
• W2021052544 cites W2118533197 @default.
• W2021052544 cites W2121487483 @default.
• W2021052544 cites W2123079914 @default.
• W2021052544 cites W2126972354 @default.
• W2021052544 cites W2128016314 @default.
• W2021052544 cites W2135488124 @default.
• W2021052544 cites W2142148410 @default.
• W2021052544 cites W2142266843 @default.
• W2021052544 cites W2143855087 @default.
• W2021052544 cites W2149880040 @default.
• W2021052544 cites W2151201664 @default.
• W2021052544 cites W2153498428 @default.
• W2021052544 cites W2153895104 @default.
• W2021052544 cites W2154662589 @default.
• W2021052544 cites W2155438446 @default.
• W2021052544 cites W2155812293 @default.
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• W2021052544 doi "https://doi.org/10.1038/sj.bjc.6605634" @default.
• W2021052544 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2856011" @default.
• W2021052544 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20354523" @default.
• W2021052544 hasPublicationYear "2010" @default.
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