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• W2021073812 abstract "The ability to control mammalian genes in a synergistic mode using synthetic transcription factors is highly desirable in fields of tissue engineering, stem cell reprogramming and fundamental research. In this study, we developed a standardized toolkit utilizing an engineered CRISPR/Cas9 system that enables customizable gene regulation in mammalian cells. The RNA-guided dCas9 protein was implemented as a programmable transcriptional activator or repressor device, including targeting of endogenous loci. For facile assembly of single or multiple CRISPR RNAs, our toolkit comprises a modular RNAimer plasmid, which encodes the required noncoding RNA components." @default.
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• W2021073812 date "2014-12-19" @default.
• W2021073812 modified "2023-09-30" @default.
• W2021073812 title "Modularized CRISPR/dCas9 Effector Toolkit for Target-Specific Gene Regulation" @default.
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• W2021073812 doi "https://doi.org/10.1021/sb500035y" @default.
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