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- W2021083314 abstract "Only three forms of Kunjin virus-specified RNA were isolated from cytoplasm early after the latent period (about 15 hr) viz., 44 S genomic-sized single-stranded RNA, 20 S double-stranded replicative form (RF), and 20-28 S partially ribonuclease-resistant (about 70%) replicative intermediate (RI). The RF and RI were resolved by electrophoresis in aqueous-agarose gel only following LiCl fractionation. The RI did not enter urea-polyacrylamide gels. After denaturation of untreated or RNase-treated RI and RF, only 44 S RNA was present in electropherograms. RNA polymerase activity at 8 hr postinfection was detected by in vitro assays of cytoplasmic extracts and reached a maximum at 24 hr, the only major labeled product being RF; a trace amount of free 44 S RNA was also produced. These results, and the kinetics of incorporation of [3H]uridine into RI, RF, and 44 S RNA in pulse and pulse-chase experiments, formed the basis of a model in which flavivirus RF functions as a recycling template for semiconservative and (mainly) asymmetric replication, on which only one nascent strand is synthesized per cycle." @default.
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- W2021083314 title "Replication strategy of Kunjin Virus: Evidence for recycling role of replicative form RNA as template in semiconservative and asymmetric replication" @default.
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- W2021083314 doi "https://doi.org/10.1016/0042-6822(85)90446-5" @default.
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