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- W2021095556 abstract "Posterior polar plasm from Binsc/B6Y cleavage embryos of Drosophila melanogaster was transferred to the mid-ventral region of mwh e4 embryos of the same age. Between 15 min and 5 h after cytoplasmic injections, embryos were analyzed at the electron microscopic level for the formation of cells with implanted cytoplasm. Characteristic subcellular organelles, the polar granules, which are exclusively located at the posterior pole of the egg, served as a morphological marker to identify the transferred cytoplasm. The appearance of round nuclear bodies, which are only present in nuclei of cells containing posterior cytoplasm, the so-called pole cells, was used as a further criterion for successful transfers. Polar granules and nuclear bodies could be found in eight and ten cells in two of the seven embryos and analyzed at the blastoderm stage. Experimentally induced pole cells were inserted among pole cells of y sn3 mal blastoderm hosts in order to determine if they could function normally and give rise to offspring. About 3% of the flies, which were derived from these embryos and mated to mwh e4 partners, had y sn3 mal germ cells as well as mwh e4 ones. In addition, germ-line mosaicism in these flies could be “visualized” by testing histochemically for aldehyde oxidase activity. Mid-ventral blastoderm cells when transferred from non-injected mwh e4 embryos to the posterior pole of y sn3 mal blastoderm embryos did not differentiate into germ cells but instead participated in the development of somatic tissues of the hosts. To find out if a pole cell experimentally induced at the mid-ventral region of the egg can be incorporated into the germ line, normal mwh e4 pole cells were implanted into this area of y sn3 mal blastoderm hosts. About 5% of the fertile flies were found to have a mosaic germ line. Our results show (1) that cytoplasm of the posterior region of the Drosophila egg contains germ-cell determinants which function autonomously after transplantation to a somatic, i.e. mid-ventral region of the egg; (2) that pole cells either formed in or transferred to this ectopic region can still reach the gonads and contribute to the germ line." @default.
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- W2021095556 title "The autonomous function of germ plasm in a somatic region of the Drosophila egg" @default.
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- W2021095556 doi "https://doi.org/10.1016/0014-4827(76)90662-5" @default.
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