FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021108969> ?p ?o ?g. }

• W2021108969 endingPage "401" @default.
• W2021108969 startingPage "389" @default.
• W2021108969 abstract "Confluent adult and fetal human glomerular epithelial cells were incubated for 24 h in the presence of [3H]-amino acids and [35S]sulfate. Two heparan-35SO4 proteoglycans were released into the culture medium. These 35S-labeled proteoglycans eluted as a single peak from anion exchange chromatographic columns, but were separable by gel filtration on Sepharose CL-6B columns. The larger heparan-35SO4 proteoglycan eluted with the column void volume and at a Kav of 0.26 from Sepharose CL-4B columns. The most abundant medium heparan-35SO4 proteoglycan was a high buoyant density proteoglycan similar in hydrodynamic size (Sepharose CL-6B Kav 0.23) to those previously described in glomerular basement membranes and isolated glomeruli. Heparan-35SO4 chains from both proteoglycans were 36 kDa. A smaller proportion of Sepharose CL-6B excluded dermatan-35SO4 proteoglycan was also synthesized by these cells. The predominant protein cores of both medium heparan-35SO4 proteoglycans were approximately 230 and 180 kDa. A hybrid chondroitin/dermatan-heparan-35SO4 proteoglycan with an 80-kDa protein core copurified with the smaller medium heparan-35SO4 proteoglycan. This 35S-labeled proteoglycan appeared as a diffuse, chondroitinase ABC sensitive 155-kDa fluorographic band in sodium dodecyl sulfate-polyacrylamide gels after the Sepharose CL-6B Kav 0.23 35S-labeled proteoglycan fraction was digested with heparitinase. The heparitinase generated heparan sulfate proteoglycan protein cores and the 155-kDa hybrid proteoglycan fragment had molecular weights similar to those previously identified in rat glomerular basement membrane and glomeruli using antibodies against a basement membrane tumor proteoglycan precursor (Klein et al. J. Cell Biol. 106, 963–970, 1988). Thus, human glomerular epithelial cells in culture are capable of synthesizing, processing, and releasing heparan sulfate proteoglycans which are similar to those synthesized in vivo and found in the glomerular basement membrane. These proteoglycans may belong to a family of related basement membrane proteoglycans." @default.
• W2021108969 created "2016-06-24" @default.
• W2021108969 creator A5014768118 @default.
• W2021108969 creator A5016662680 @default.
• W2021108969 creator A5024470459 @default.
• W2021108969 creator A5030449236 @default.
• W2021108969 creator A5053935954 @default.
• W2021108969 creator A5076638620 @default.
• W2021108969 date "1990-03-01" @default.
• W2021108969 modified "2023-09-23" @default.
• W2021108969 title "Partial characterization of proteoglycans synthesized by human glomerular epithelial cells in culture" @default.
• W2021108969 cites W137187997 @default.
• W2021108969 cites W1488050272 @default.
• W2021108969 cites W1531518952 @default.
• W2021108969 cites W1548997028 @default.
• W2021108969 cites W1563321047 @default.
• W2021108969 cites W1581375878 @default.
• W2021108969 cites W159584199 @default.
• W2021108969 cites W1596577347 @default.
• W2021108969 cites W1598185076 @default.
• W2021108969 cites W1601915458 @default.
• W2021108969 cites W1814081193 @default.
• W2021108969 cites W1975364338 @default.
• W2021108969 cites W1998741154 @default.
• W2021108969 cites W2001534689 @default.
• W2021108969 cites W2004369793 @default.
• W2021108969 cites W2031234071 @default.
• W2021108969 cites W2064386269 @default.
• W2021108969 cites W2067422836 @default.
• W2021108969 cites W2081189780 @default.
• W2021108969 cites W2082503226 @default.
• W2021108969 cites W2085283016 @default.
• W2021108969 cites W2086501529 @default.
• W2021108969 cites W2088606047 @default.
• W2021108969 cites W2089691974 @default.
• W2021108969 cites W2100837269 @default.
• W2021108969 cites W2110469556 @default.
• W2021108969 cites W2114138321 @default.
• W2021108969 cites W2114950491 @default.
• W2021108969 cites W2123442023 @default.
• W2021108969 cites W2131042297 @default.
• W2021108969 cites W2135845349 @default.
• W2021108969 cites W2139643413 @default.
• W2021108969 cites W2148870351 @default.
• W2021108969 cites W2150912407 @default.
• W2021108969 cites W2151084936 @default.
• W2021108969 cites W2331591746 @default.
• W2021108969 cites W4256468456 @default.
• W2021108969 cites W4361867811 @default.
• W2021108969 cites W91915837 @default.
• W2021108969 doi "https://doi.org/10.1016/0003-9861(90)90595-p" @default.
• W2021108969 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2106832" @default.
• W2021108969 hasPublicationYear "1990" @default.
• W2021108969 type Work @default.
• W2021108969 sameAs 2021108969 @default.
• W2021108969 citedByCount "22" @default.
• W2021108969 countsByYear W20211089692012 @default.
• W2021108969 crossrefType "journal-article" @default.
• W2021108969 hasAuthorship W2021108969A5014768118 @default.
• W2021108969 hasAuthorship W2021108969A5016662680 @default.
• W2021108969 hasAuthorship W2021108969A5024470459 @default.
• W2021108969 hasAuthorship W2021108969A5030449236 @default.
• W2021108969 hasAuthorship W2021108969A5053935954 @default.
• W2021108969 hasAuthorship W2021108969A5076638620 @default.
• W2021108969 hasConcept C116084860 @default.
• W2021108969 hasConcept C153074725 @default.
• W2021108969 hasConcept C181199279 @default.
• W2021108969 hasConcept C185592680 @default.
• W2021108969 hasConcept C189165786 @default.
• W2021108969 hasConcept C22731427 @default.
• W2021108969 hasConcept C2775895372 @default.
• W2021108969 hasConcept C2776193487 @default.
• W2021108969 hasConcept C2778041096 @default.
• W2021108969 hasConcept C2778066728 @default.
• W2021108969 hasConcept C2779335624 @default.
• W2021108969 hasConcept C2779881004 @default.
• W2021108969 hasConcept C55493867 @default.
• W2021108969 hasConcept C86803240 @default.
• W2021108969 hasConcept C95444343 @default.
• W2021108969 hasConceptScore W2021108969C116084860 @default.
• W2021108969 hasConceptScore W2021108969C153074725 @default.
• W2021108969 hasConceptScore W2021108969C181199279 @default.
• W2021108969 hasConceptScore W2021108969C185592680 @default.
• W2021108969 hasConceptScore W2021108969C189165786 @default.
• W2021108969 hasConceptScore W2021108969C22731427 @default.
• W2021108969 hasConceptScore W2021108969C2775895372 @default.
• W2021108969 hasConceptScore W2021108969C2776193487 @default.
• W2021108969 hasConceptScore W2021108969C2778041096 @default.
• W2021108969 hasConceptScore W2021108969C2778066728 @default.
• W2021108969 hasConceptScore W2021108969C2779335624 @default.
• W2021108969 hasConceptScore W2021108969C2779881004 @default.
• W2021108969 hasConceptScore W2021108969C55493867 @default.
• W2021108969 hasConceptScore W2021108969C86803240 @default.
• W2021108969 hasConceptScore W2021108969C95444343 @default.
• W2021108969 hasIssue "2" @default.
• W2021108969 hasLocation W20211089691 @default.
• W2021108969 hasLocation W20211089692 @default.
• W2021108969 hasOpenAccess W2021108969 @default.

• next