Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021136660> ?p ?o ?g. }
- W2021136660 endingPage "3820" @default.
- W2021136660 startingPage "3811" @default.
- W2021136660 abstract "Trypanosoma brucei is a member of the early-diverged, protistan family Trypanosomatidae and a lethal parasite causing African Sleeping Sickness in humans. Recent studies revealed that T. brucei harbors extremely divergent orthologues of the general transcription factors TBP, TFIIA, TFIIB and TFIIH and showed that these factors are essential for initiating RNA polymerase II-mediated synthesis of spliced leader (SL) RNA, a trans splicing substrate and key molecule in trypanosome mRNA maturation. In yeast and metazoans, TFIIH is composed of a core of seven conserved subunits and the ternary cyclin-activating kinase (CAK) complex. Conversely, only four TFIIH subunits have been identified in T. brucei. Here, we characterize the first protistan TFIIH which was purified in its transcriptionally active form from T. brucei extracts. The complex consisted of all seven core subunits but lacked the CAK sub-complex; instead it contained two trypanosomatid-specific subunits, which were indispensable for parasite viability and SL RNA gene transcription. These findings were corroborated by comparing the molecular structures of trypanosome and human TFIIH. While the ring-shaped core domain was surprisingly congruent between the two structures, trypanosome TFIIH lacked the knob-like CAK moiety and exhibited extra densities on either side of the ring, presumably due to the specific subunits." @default.
- W2021136660 created "2016-06-24" @default.
- W2021136660 creator A5023500801 @default.
- W2021136660 creator A5044396203 @default.
- W2021136660 creator A5050008313 @default.
- W2021136660 date "2009-04-22" @default.
- W2021136660 modified "2023-09-30" @default.
- W2021136660 title "Transcriptionally active TFIIH of the early-diverged eukaryote Trypanosoma brucei harbors two novel core subunits but not a cyclin-activating kinase complex" @default.
- W2021136660 cites W1545169951 @default.
- W2021136660 cites W1894335898 @default.
- W2021136660 cites W1967288647 @default.
- W2021136660 cites W1974906822 @default.
- W2021136660 cites W1978570354 @default.
- W2021136660 cites W1989150820 @default.
- W2021136660 cites W2003482071 @default.
- W2021136660 cites W2023560657 @default.
- W2021136660 cites W2025571437 @default.
- W2021136660 cites W2038543938 @default.
- W2021136660 cites W2041757320 @default.
- W2021136660 cites W2045828490 @default.
- W2021136660 cites W2050489815 @default.
- W2021136660 cites W2053341447 @default.
- W2021136660 cites W2058353382 @default.
- W2021136660 cites W2059383371 @default.
- W2021136660 cites W2064066079 @default.
- W2021136660 cites W2069675793 @default.
- W2021136660 cites W2080914451 @default.
- W2021136660 cites W2096985831 @default.
- W2021136660 cites W2099775990 @default.
- W2021136660 cites W2106810495 @default.
- W2021136660 cites W2110102880 @default.
- W2021136660 cites W2116350092 @default.
- W2021136660 cites W2116888204 @default.
- W2021136660 cites W2119326760 @default.
- W2021136660 cites W2119803020 @default.
- W2021136660 cites W2128767091 @default.
- W2021136660 cites W2132629607 @default.
- W2021136660 cites W2137015675 @default.
- W2021136660 cites W2140700237 @default.
- W2021136660 cites W2142528445 @default.
- W2021136660 cites W2147372875 @default.
- W2021136660 cites W2148668495 @default.
- W2021136660 cites W2148980809 @default.
- W2021136660 cites W2153992958 @default.
- W2021136660 cites W2154077605 @default.
- W2021136660 cites W2158208260 @default.
- W2021136660 cites W2166530501 @default.
- W2021136660 cites W2168799765 @default.
- W2021136660 cites W2169615432 @default.
- W2021136660 cites W2170991287 @default.
- W2021136660 doi "https://doi.org/10.1093/nar/gkp236" @default.
- W2021136660 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2699521" @default.
- W2021136660 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19386623" @default.
- W2021136660 hasPublicationYear "2009" @default.
- W2021136660 type Work @default.
- W2021136660 sameAs 2021136660 @default.
- W2021136660 citedByCount "41" @default.
- W2021136660 countsByYear W20211366602012 @default.
- W2021136660 countsByYear W20211366602013 @default.
- W2021136660 countsByYear W20211366602014 @default.
- W2021136660 countsByYear W20211366602015 @default.
- W2021136660 countsByYear W20211366602016 @default.
- W2021136660 countsByYear W20211366602017 @default.
- W2021136660 countsByYear W20211366602018 @default.
- W2021136660 countsByYear W20211366602019 @default.
- W2021136660 countsByYear W20211366602020 @default.
- W2021136660 countsByYear W20211366602021 @default.
- W2021136660 countsByYear W20211366602023 @default.
- W2021136660 crossrefType "journal-article" @default.
- W2021136660 hasAuthorship W2021136660A5023500801 @default.
- W2021136660 hasAuthorship W2021136660A5044396203 @default.
- W2021136660 hasAuthorship W2021136660A5050008313 @default.
- W2021136660 hasBestOaLocation W20211366601 @default.
- W2021136660 hasConcept C101762097 @default.
- W2021136660 hasConcept C104317684 @default.
- W2021136660 hasConcept C104451858 @default.
- W2021136660 hasConcept C134935766 @default.
- W2021136660 hasConcept C138885662 @default.
- W2021136660 hasConcept C150194340 @default.
- W2021136660 hasConcept C179926584 @default.
- W2021136660 hasConcept C188740287 @default.
- W2021136660 hasConcept C2779502636 @default.
- W2021136660 hasConcept C41895202 @default.
- W2021136660 hasConcept C44498088 @default.
- W2021136660 hasConcept C54355233 @default.
- W2021136660 hasConcept C56149667 @default.
- W2021136660 hasConcept C64350747 @default.
- W2021136660 hasConcept C67705224 @default.
- W2021136660 hasConcept C86803240 @default.
- W2021136660 hasConcept C95444343 @default.
- W2021136660 hasConceptScore W2021136660C101762097 @default.
- W2021136660 hasConceptScore W2021136660C104317684 @default.
- W2021136660 hasConceptScore W2021136660C104451858 @default.
- W2021136660 hasConceptScore W2021136660C134935766 @default.
- W2021136660 hasConceptScore W2021136660C138885662 @default.
- W2021136660 hasConceptScore W2021136660C150194340 @default.
- W2021136660 hasConceptScore W2021136660C179926584 @default.
- W2021136660 hasConceptScore W2021136660C188740287 @default.