Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021143003> ?p ?o ?g. }
- W2021143003 endingPage "2193" @default.
- W2021143003 startingPage "2187" @default.
- W2021143003 abstract "Direct mass spectrometric quantification of peptides and proteins is compromised by the wide variabilities in ionization efficiency which are hallmarks of both the MALDI and ESI ionization techniques. We describe here the implementation of a fluorescence detection system for measurement of the UV-excited intrinsic fluorescence (UV-IF) from peptides and proteins just prior to their exit and electrospray ionization from an ESI capillary. The fluorescence signal provides a quantifiable measure of the amount of protein or peptide present, while direct or tandem mass spectrometric analysis (MS/MS) on the ESI-generated ions provides information on identity. We fabricated an inexpensive, modular fluorescence excitation and detection device utilizing an ultraviolet light-emitting diode for excitation in a ∼300 nL fluorescence detection cell integrated into the fused-silica separation column. The fluorescence signal is linear over 3 orders of magnitude with on-column limits of detection in the low femtomole range. Chromatographically separated intact proteins analyzed using UV-IF prior to top-down mass spectrometry demonstrated sensitive detection of proteins as large as 77 kDa." @default.
- W2021143003 created "2016-06-24" @default.
- W2021143003 creator A5005583559 @default.
- W2021143003 creator A5006295902 @default.
- W2021143003 creator A5006873729 @default.
- W2021143003 creator A5021884776 @default.
- W2021143003 creator A5022080753 @default.
- W2021143003 creator A5036483354 @default.
- W2021143003 creator A5049408844 @default.
- W2021143003 creator A5086840789 @default.
- W2021143003 date "2011-02-11" @default.
- W2021143003 modified "2023-09-27" @default.
- W2021143003 title "Parallel Detection of Intrinsic Fluorescence from Peptides and Proteins for Quantification during Mass Spectrometric Analysis" @default.
- W2021143003 cites W1083984924 @default.
- W2021143003 cites W1587423451 @default.
- W2021143003 cites W1987208202 @default.
- W2021143003 cites W1989636155 @default.
- W2021143003 cites W1992403473 @default.
- W2021143003 cites W2004394975 @default.
- W2021143003 cites W2008900802 @default.
- W2021143003 cites W2013909009 @default.
- W2021143003 cites W2019989581 @default.
- W2021143003 cites W2022250239 @default.
- W2021143003 cites W2023096047 @default.
- W2021143003 cites W2024921442 @default.
- W2021143003 cites W2029797741 @default.
- W2021143003 cites W2031884770 @default.
- W2021143003 cites W2031909006 @default.
- W2021143003 cites W2033574710 @default.
- W2021143003 cites W2041651069 @default.
- W2021143003 cites W2042334208 @default.
- W2021143003 cites W2043727128 @default.
- W2021143003 cites W2047216280 @default.
- W2021143003 cites W2059976302 @default.
- W2021143003 cites W2062012547 @default.
- W2021143003 cites W2063159270 @default.
- W2021143003 cites W2064862042 @default.
- W2021143003 cites W2067519806 @default.
- W2021143003 cites W2068481474 @default.
- W2021143003 cites W2072118639 @default.
- W2021143003 cites W2074876700 @default.
- W2021143003 cites W2079449211 @default.
- W2021143003 cites W2079634693 @default.
- W2021143003 cites W2082461846 @default.
- W2021143003 cites W2086512939 @default.
- W2021143003 cites W2088687602 @default.
- W2021143003 cites W2090048784 @default.
- W2021143003 cites W2096057003 @default.
- W2021143003 cites W2102329829 @default.
- W2021143003 cites W2110412954 @default.
- W2021143003 cites W2117327695 @default.
- W2021143003 cites W2123361937 @default.
- W2021143003 cites W2137902859 @default.
- W2021143003 cites W2144948497 @default.
- W2021143003 cites W2155935626 @default.
- W2021143003 cites W2161574105 @default.
- W2021143003 cites W2318705632 @default.
- W2021143003 cites W2331495629 @default.
- W2021143003 cites W2491458126 @default.
- W2021143003 doi "https://doi.org/10.1021/ac103023q" @default.
- W2021143003 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3056920" @default.
- W2021143003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21314137" @default.
- W2021143003 hasPublicationYear "2011" @default.
- W2021143003 type Work @default.
- W2021143003 sameAs 2021143003 @default.
- W2021143003 citedByCount "16" @default.
- W2021143003 countsByYear W20211430032012 @default.
- W2021143003 countsByYear W20211430032013 @default.
- W2021143003 countsByYear W20211430032014 @default.
- W2021143003 countsByYear W20211430032015 @default.
- W2021143003 countsByYear W20211430032016 @default.
- W2021143003 countsByYear W20211430032017 @default.
- W2021143003 countsByYear W20211430032018 @default.
- W2021143003 countsByYear W20211430032020 @default.
- W2021143003 countsByYear W20211430032022 @default.
- W2021143003 crossrefType "journal-article" @default.
- W2021143003 hasAuthorship W2021143003A5005583559 @default.
- W2021143003 hasAuthorship W2021143003A5006295902 @default.
- W2021143003 hasAuthorship W2021143003A5006873729 @default.
- W2021143003 hasAuthorship W2021143003A5021884776 @default.
- W2021143003 hasAuthorship W2021143003A5022080753 @default.
- W2021143003 hasAuthorship W2021143003A5036483354 @default.
- W2021143003 hasAuthorship W2021143003A5049408844 @default.
- W2021143003 hasAuthorship W2021143003A5086840789 @default.
- W2021143003 hasBestOaLocation W20211430032 @default.
- W2021143003 hasConcept C113196181 @default.
- W2021143003 hasConcept C119128265 @default.
- W2021143003 hasConcept C121332964 @default.
- W2021143003 hasConcept C1342733 @default.
- W2021143003 hasConcept C158711907 @default.
- W2021143003 hasConcept C162356407 @default.
- W2021143003 hasConcept C185592680 @default.
- W2021143003 hasConcept C194451485 @default.
- W2021143003 hasConcept C202157981 @default.
- W2021143003 hasConcept C31827203 @default.
- W2021143003 hasConcept C40684141 @default.
- W2021143003 hasConcept C43617362 @default.
- W2021143003 hasConcept C62520636 @default.