Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021146258> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2021146258 abstract "In the past the effects of laser irradiation upon tissue have been assessed based on structural and macroscopic characteristics such as temperature, pressure and tissue mass removal. However, the effects of laser irradiation on a cellular level are not well understood and it is postulated that cellular injury caused by laser treatment may affect the efficacy of the laser procedure. In this research we have used an alternative method of detecting injury by targeting the heat shock protein (Hsp70). A stable cell line was generated containing the luciferase reporter gene attached to the heat shock protein (Hsp70). After thermal injury luciferase is produced in tandem with the heat shock protein to emit bioluminescence at 563 nm. The luminescence was quantified with a photon counting ICCD camera system. The heat shock to bring about Hsp70 transcription was created by immersing the cells in a water bath or by irradiating the cells with a Holmium:YAG pulsed laser (λ= 2.1 μm, τ p = 250 μs). For the laser experiments, radiant exposures varied from 5 to 30 mJ/mm2 and the number of pulses varied at 15, 25 and 35. The peak expression of luciferase was found to be 3 to 4 hours post heat shock for lower exposures but increase to between 6 and 9 hours if higher radiant energies are used. An experiment was also done to assess to what extent the cellular response to heat followed the Arrhenius rate process.© (2001) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only." @default.
- W2021146258 created "2016-06-24" @default.
- W2021146258 creator A5004098308 @default.
- W2021146258 creator A5025502776 @default.
- W2021146258 creator A5033954704 @default.
- W2021146258 creator A5034826457 @default.
- W2021146258 date "2001-07-09" @default.
- W2021146258 modified "2023-09-23" @default.
- W2021146258 title "Optical imaging of Hsp70 gene expression following thermal laser injury" @default.
- W2021146258 doi "https://doi.org/10.1117/12.434723" @default.
- W2021146258 hasPublicationYear "2001" @default.
- W2021146258 type Work @default.
- W2021146258 sameAs 2021146258 @default.
- W2021146258 citedByCount "1" @default.
- W2021146258 crossrefType "proceedings-article" @default.
- W2021146258 hasAuthorship W2021146258A5004098308 @default.
- W2021146258 hasAuthorship W2021146258A5025502776 @default.
- W2021146258 hasAuthorship W2021146258A5033954704 @default.
- W2021146258 hasAuthorship W2021146258A5034826457 @default.
- W2021146258 hasConcept C104317684 @default.
- W2021146258 hasConcept C111335760 @default.
- W2021146258 hasConcept C111337013 @default.
- W2021146258 hasConcept C120665830 @default.
- W2021146258 hasConcept C121332964 @default.
- W2021146258 hasConcept C12554922 @default.
- W2021146258 hasConcept C137984847 @default.
- W2021146258 hasConcept C148869448 @default.
- W2021146258 hasConcept C153911025 @default.
- W2021146258 hasConcept C161997846 @default.
- W2021146258 hasConcept C185544564 @default.
- W2021146258 hasConcept C185592680 @default.
- W2021146258 hasConcept C192562407 @default.
- W2021146258 hasConcept C205260736 @default.
- W2021146258 hasConcept C49040817 @default.
- W2021146258 hasConcept C5176359 @default.
- W2021146258 hasConcept C520434653 @default.
- W2021146258 hasConcept C54009773 @default.
- W2021146258 hasConcept C55493867 @default.
- W2021146258 hasConcept C68991219 @default.
- W2021146258 hasConcept C86803240 @default.
- W2021146258 hasConcept C95444343 @default.
- W2021146258 hasConceptScore W2021146258C104317684 @default.
- W2021146258 hasConceptScore W2021146258C111335760 @default.
- W2021146258 hasConceptScore W2021146258C111337013 @default.
- W2021146258 hasConceptScore W2021146258C120665830 @default.
- W2021146258 hasConceptScore W2021146258C121332964 @default.
- W2021146258 hasConceptScore W2021146258C12554922 @default.
- W2021146258 hasConceptScore W2021146258C137984847 @default.
- W2021146258 hasConceptScore W2021146258C148869448 @default.
- W2021146258 hasConceptScore W2021146258C153911025 @default.
- W2021146258 hasConceptScore W2021146258C161997846 @default.
- W2021146258 hasConceptScore W2021146258C185544564 @default.
- W2021146258 hasConceptScore W2021146258C185592680 @default.
- W2021146258 hasConceptScore W2021146258C192562407 @default.
- W2021146258 hasConceptScore W2021146258C205260736 @default.
- W2021146258 hasConceptScore W2021146258C49040817 @default.
- W2021146258 hasConceptScore W2021146258C5176359 @default.
- W2021146258 hasConceptScore W2021146258C520434653 @default.
- W2021146258 hasConceptScore W2021146258C54009773 @default.
- W2021146258 hasConceptScore W2021146258C55493867 @default.
- W2021146258 hasConceptScore W2021146258C68991219 @default.
- W2021146258 hasConceptScore W2021146258C86803240 @default.
- W2021146258 hasConceptScore W2021146258C95444343 @default.
- W2021146258 hasLocation W20211462581 @default.
- W2021146258 hasOpenAccess W2021146258 @default.
- W2021146258 hasPrimaryLocation W20211462581 @default.
- W2021146258 hasRelatedWork W1923695016 @default.
- W2021146258 hasRelatedWork W1978864156 @default.
- W2021146258 hasRelatedWork W1980701376 @default.
- W2021146258 hasRelatedWork W2000731082 @default.
- W2021146258 hasRelatedWork W2032542278 @default.
- W2021146258 hasRelatedWork W2043055347 @default.
- W2021146258 hasRelatedWork W2050187359 @default.
- W2021146258 hasRelatedWork W2054031946 @default.
- W2021146258 hasRelatedWork W2058609079 @default.
- W2021146258 hasRelatedWork W2073296671 @default.
- W2021146258 hasRelatedWork W2073355076 @default.
- W2021146258 hasRelatedWork W2091731099 @default.
- W2021146258 hasRelatedWork W2095508808 @default.
- W2021146258 hasRelatedWork W2128705181 @default.
- W2021146258 hasRelatedWork W2133257760 @default.
- W2021146258 hasRelatedWork W2418453724 @default.
- W2021146258 hasRelatedWork W2534053604 @default.
- W2021146258 hasRelatedWork W2787281466 @default.
- W2021146258 hasRelatedWork W3008504394 @default.
- W2021146258 hasRelatedWork W245517343 @default.
- W2021146258 isParatext "false" @default.
- W2021146258 isRetracted "false" @default.
- W2021146258 magId "2021146258" @default.
- W2021146258 workType "article" @default.