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• W2021148637 abstract "The present study examined whether lysophosphatidic acid (LPA) induces histamine release and the role of Rho-associated protein kinase (ROCK) in histamine release utilizing Y-27632, an inhibitor of ROCK. Rat peritoneal mast cells and mouse skin fragments were challenged with LPA; subsequently, histamine contents were measured by spectrofluorometric assay. LPA-induced histamine release from mast cells and skin fragments occurred in a time- and dose-dependent manner Pretreatment with Y-27632 inhibited LPA-induced histamine release in a dose-dependent fashion. LPA-induced histamine release was not influenced by calcium-free conditions; however, A23187-induced histamine release decreased significantly. TMB-8, an intracellular calcium antagonist, dose-dependently inhibited the histamine release under these conditions. Additionally, Y-27632 scarcely affected A23187-induced histamine release. These findings suggest that LPA-induced histamine release may be attributable to calcium release from intracellular stores. Moreover, ROCK participates in the extracellular calcium-independent process of LPA-induced histamine release." @default.
• W2021148637 created "2016-06-24" @default.
• W2021148637 creator A5030805351 @default.
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• W2021148637 date "2005-01-01" @default.
• W2021148637 modified "2023-10-16" @default.
• W2021148637 title "Lysophosphatidic Acid Induces Histamine Release from Mast Cells and Skin Fragments" @default.
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• W2021148637 doi "https://doi.org/10.1159/000085784" @default.
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