FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021193191> ?p ?o ?g. }

• W2021193191 endingPage "389" @default.
• W2021193191 startingPage "380" @default.
• W2021193191 abstract "We previously showed that human corneal epithelial cells (HCECs) express Toll-like receptors (TLRs), which recognize Gram-positive bacteria and respond to Staphylococcus aureus infection by the expression and secretion of proinflammatory cytokines and β-defensin-2 (hBD2). In this study, we further elucidated the underlying mechanisms regulating hBD-2 expression and its role in innate defense in HCECs in response to S. aureus challenge. Exposure of HUCL cells, a telomerase-immortalized HCEC line, to S. aureus, its exoproducts (1:10 dilution), or synthetic lipopeptide Pam3Cys (10 μg/ml) resulted in the up-regulation of hBD-2, but not hBD1 and hBD3. Similar to HUCL cells, primary HCECs responded to S. aureus-exoproducts and Pam3Cys challenge by expressing hBD2 mRNA and secreting hBD2 into the culture media. Furthermore, these stimuli induced the expression of TLR2 at both mRNA and protein levels. Consistently with its role as a major pattern-recognizing receptor, TLR2 was located at the cell surface by cell surface biotinylation. The treatment of HUCL cells with TLR2 neutralizing antibody resulted in a significant decrease in Pam3Cys-induced hBD2 production as well as IL-6, IL-8, and TNF-α secretion. The Pam3Cys-induced hBD2 expression was completely blocked by NF-κB inhibitors and partially inhibited by p38 MAP kinase and the JNK inhibitors. Conditioned media derived from HCECs challenged with S. aureus-exoproducts or Pam3Cys exhibited antibacterial activity against S. aureus, Pseudomonas aeruginosa and Escherichia coli. These findings suggest that S. aureus induces hBD2 production through TLR2-mediated pathways in HCECs and that pathogen-challenged, TLR-activated HCECs possess antimicrobial activity. Thus, the epithelium might play a role in innate defense against bacterial infection by directly killing bacteria in the cornea." @default.
• W2021193191 created "2016-06-24" @default.
• W2021193191 creator A5017947172 @default.
• W2021193191 creator A5033377592 @default.
• W2021193191 creator A5068641506 @default.
• W2021193191 date "2006-02-01" @default.
• W2021193191 modified "2023-09-28" @default.
• W2021193191 title "Toll-like receptor 2-mediated expression of β-defensin-2 in human corneal epithelial cells" @default.
• W2021193191 cites W1536952772 @default.
• W2021193191 cites W1571292455 @default.
• W2021193191 cites W1622881476 @default.
• W2021193191 cites W1655860314 @default.
• W2021193191 cites W1765362282 @default.
• W2021193191 cites W176879792 @default.
• W2021193191 cites W1863323524 @default.
• W2021193191 cites W1902083634 @default.
• W2021193191 cites W1951587871 @default.
• W2021193191 cites W1953329867 @default.
• W2021193191 cites W1963658902 @default.
• W2021193191 cites W1968931227 @default.
• W2021193191 cites W1969457486 @default.
• W2021193191 cites W1972528203 @default.
• W2021193191 cites W1980001920 @default.
• W2021193191 cites W1981318671 @default.
• W2021193191 cites W1986090702 @default.
• W2021193191 cites W1986520266 @default.
• W2021193191 cites W1990519548 @default.
• W2021193191 cites W2003738186 @default.
• W2021193191 cites W2004465286 @default.
• W2021193191 cites W2009528488 @default.
• W2021193191 cites W2013621172 @default.
• W2021193191 cites W2014538073 @default.
• W2021193191 cites W2014941940 @default.
• W2021193191 cites W2019039210 @default.
• W2021193191 cites W2020234721 @default.
• W2021193191 cites W2020922034 @default.
• W2021193191 cites W2025983848 @default.
• W2021193191 cites W2038542042 @default.
• W2021193191 cites W2042092530 @default.
• W2021193191 cites W2046573677 @default.
• W2021193191 cites W2049504005 @default.
• W2021193191 cites W2053793434 @default.
• W2021193191 cites W2054720087 @default.
• W2021193191 cites W2057198053 @default.
• W2021193191 cites W2066460916 @default.
• W2021193191 cites W2081347555 @default.
• W2021193191 cites W2081756009 @default.
• W2021193191 cites W2082057885 @default.
• W2021193191 cites W2087793279 @default.
• W2021193191 cites W2090042814 @default.
• W2021193191 cites W2093412162 @default.
• W2021193191 cites W2095344941 @default.
• W2021193191 cites W2101363646 @default.
• W2021193191 cites W2108105410 @default.
• W2021193191 cites W2116236493 @default.
• W2021193191 cites W2120046045 @default.
• W2021193191 cites W2121949549 @default.
• W2021193191 cites W2122708604 @default.
• W2021193191 cites W2124924168 @default.
• W2021193191 cites W2125935614 @default.
• W2021193191 cites W2125985831 @default.
• W2021193191 cites W2127988591 @default.
• W2021193191 cites W2128315833 @default.
• W2021193191 cites W2130946949 @default.
• W2021193191 cites W2133449490 @default.
• W2021193191 cites W2140015861 @default.
• W2021193191 cites W2143635657 @default.
• W2021193191 cites W2146994866 @default.
• W2021193191 cites W2151691579 @default.
• W2021193191 cites W2153324964 @default.
• W2021193191 cites W2160783859 @default.
• W2021193191 cites W2163665267 @default.
• W2021193191 cites W2167059596 @default.
• W2021193191 cites W2170442599 @default.
• W2021193191 cites W4313309903 @default.
• W2021193191 doi "https://doi.org/10.1016/j.micinf.2005.07.006" @default.
• W2021193191 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2666383" @default.
• W2021193191 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16242370" @default.
• W2021193191 hasPublicationYear "2006" @default.
• W2021193191 type Work @default.
• W2021193191 sameAs 2021193191 @default.
• W2021193191 citedByCount "119" @default.
• W2021193191 countsByYear W20211931912012 @default.
• W2021193191 countsByYear W20211931912013 @default.
• W2021193191 countsByYear W20211931912014 @default.
• W2021193191 countsByYear W20211931912015 @default.
• W2021193191 countsByYear W20211931912016 @default.
• W2021193191 countsByYear W20211931912017 @default.
• W2021193191 countsByYear W20211931912018 @default.
• W2021193191 countsByYear W20211931912019 @default.
• W2021193191 countsByYear W20211931912020 @default.
• W2021193191 countsByYear W20211931912021 @default.
• W2021193191 countsByYear W20211931912022 @default.
• W2021193191 countsByYear W20211931912023 @default.
• W2021193191 crossrefType "journal-article" @default.
• W2021193191 hasAuthorship W2021193191A5017947172 @default.
• W2021193191 hasAuthorship W2021193191A5033377592 @default.
• W2021193191 hasAuthorship W2021193191A5068641506 @default.

• next