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- W2021194242 abstract "A method employing immunomagnetic beads was developed for the isolation of Escherichia coli O157:H7. Magnetic beads precoated with sheep anti-rabbit IgG were coated with rabbit antiserum against E. coli O157. The level of bacteria, amount of beads, amount of antibody coating the beads and incubation time were varied to determine their effects on recovery of the organism. The sensitivity of the test for recovery of E. coli O157:H7 was 1 × 101 cfu ml−1. The organism was selectively recovered from 24 h enrichment cultures of meat products in which the initial inoculum level was 1 bacterium g−1. Sorbitol negative colonies were selected from Sorbitol Mac-Conkey agar and were positively identified as E. coli using a direct fluorescent antibody procedure. The results indicate that immunomagnetic separation is a convenient and rapid method for the isolation of E. coli O157:H7 from foods." @default.
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- W2021194242 date "1992-06-01" @default.
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- W2021194242 title "Rapid isolation of Escherichia coli O157:H7 from enrichment cultures of foods using an immunomagnetic separation method" @default.
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- W2021194242 doi "https://doi.org/10.1016/0740-0020(92)80018-y" @default.
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