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• W2021199615 abstract "Human peripheral blood mononuclear leukocytes were found to produce large amounts of interferon-gamma in response to the calcium ionophore A23187 combined with the phorbol ester mezerein. This effect was synergistic since A23187 and mezerein separately did not induce significant interferon-gamma levels. A23187 plus mezerein induced high levels of interleukin-2 and interferon-gamma mRNAs. The presence of cyclohexamide, which inhibits protein synthesis by greater than 99%, had no effect on the induction by A23187 plus meserein of mRNA from either gene, indicating that synthesis of proteins such as interleukin-2 is not required for activation of either gene. In addition, using different protocols of stimulation of lymphocytes it was shown that both interleukin-2 and interferon-gamma mRNA steady state levels varied in concert. We conclude that interferon-gamma and interleukin-2 genes are coordinately expressed and activation of transcription of these genes is a primary event of T cell activation independent of production of other proteins." @default.
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• W2021199615 date "1987-08-01" @default.
• W2021199615 modified "2023-09-25" @default.
• W2021199615 title "The induction of IFN-gamma production and m-RNAs of interleukin 2 and IFN-gamma by phorbol esters and a calcium ionophore" @default.
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• W2021199615 doi "https://doi.org/10.1016/0006-291x(87)90735-2" @default.
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