FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021213362> ?p ?o ?g. }

• W2021213362 endingPage "665" @default.
• W2021213362 startingPage "658" @default.
• W2021213362 abstract "Using the Cre/lox recombination system, we analyzed the extent to which T-DNA transfer to the plant cell and T-DNA integration into the plant genome determine the transformation and cotransformation frequencies of Arabidopsis root cells. Without selection for transformation competence, the stable transformation frequency of shoots obtained after cocultivation and regeneration on nonselective medium is below 0.5%. T-DNA transfer and expression occur in 5% of the shoots, indicating that the T-DNA integrates in less than 10% of the transiently expressing plant cells. A limited fraction of root cells, predominantly located at the wounded sites and in the pericycle, are competent for interaction with agrobacteria and the uptake of a T-DNA, as demonstrated by histochemical GUS staining. When selection for transformation competence is applied, the picture is completely different. Then, approximately 50% of the transformants show transient expression of a second, nonselected T-DNA and almost 50% of these cotransferred T-DNAs are integrated into the plant genome. Our results indicate that both T-DNA transfer and T-DNA integration limit the transformation and cotransformation frequencies and that plant cell competence for transformation is based on these two factors." @default.
• W2021213362 created "2016-06-24" @default.
• W2021213362 creator A5033772999 @default.
• W2021213362 creator A5046727928 @default.
• W2021213362 creator A5047966980 @default.
• W2021213362 creator A5090717064 @default.
• W2021213362 date "2000-06-01" @default.
• W2021213362 modified "2023-10-14" @default.
• W2021213362 title "Determination of the T-DNA Transfer and the T-DNA Integration Frequencies upon Cocultivation of <i>Arabidopsis thaliana</i> Root Explants" @default.
• W2021213362 cites W117352546 @default.
• W2021213362 cites W1210145542 @default.
• W2021213362 cites W123038640 @default.
• W2021213362 cites W123598076 @default.
• W2021213362 cites W1539503448 @default.
• W2021213362 cites W1583766297 @default.
• W2021213362 cites W1595244129 @default.
• W2021213362 cites W1595851036 @default.
• W2021213362 cites W1975327433 @default.
• W2021213362 cites W1976391864 @default.
• W2021213362 cites W1984215496 @default.
• W2021213362 cites W1989016869 @default.
• W2021213362 cites W1989557734 @default.
• W2021213362 cites W1990849226 @default.
• W2021213362 cites W1991219046 @default.
• W2021213362 cites W1994429618 @default.
• W2021213362 cites W1998963945 @default.
• W2021213362 cites W2009087758 @default.
• W2021213362 cites W2025064188 @default.
• W2021213362 cites W2025268779 @default.
• W2021213362 cites W2026272271 @default.
• W2021213362 cites W2027477511 @default.
• W2021213362 cites W2046340447 @default.
• W2021213362 cites W2067757665 @default.
• W2021213362 cites W2075437498 @default.
• W2021213362 cites W2079753315 @default.
• W2021213362 cites W2081190566 @default.
• W2021213362 cites W2084541539 @default.
• W2021213362 cites W2087918598 @default.
• W2021213362 cites W2098509576 @default.
• W2021213362 cites W2128315272 @default.
• W2021213362 cites W2147632005 @default.
• W2021213362 cites W2147734788 @default.
• W2021213362 cites W2168097853 @default.
• W2021213362 cites W2619783735 @default.
• W2021213362 cites W27207012 @default.
• W2021213362 cites W76364628 @default.
• W2021213362 doi "https://doi.org/10.1094/mpmi.2000.13.6.658" @default.
• W2021213362 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10830265" @default.
• W2021213362 hasPublicationYear "2000" @default.
• W2021213362 type Work @default.
• W2021213362 sameAs 2021213362 @default.
• W2021213362 citedByCount "62" @default.
• W2021213362 countsByYear W20212133622012 @default.
• W2021213362 countsByYear W20212133622013 @default.
• W2021213362 countsByYear W20212133622014 @default.
• W2021213362 countsByYear W20212133622016 @default.
• W2021213362 countsByYear W20212133622017 @default.
• W2021213362 countsByYear W20212133622018 @default.
• W2021213362 countsByYear W20212133622019 @default.
• W2021213362 countsByYear W20212133622020 @default.
• W2021213362 countsByYear W20212133622022 @default.
• W2021213362 crossrefType "journal-article" @default.
• W2021213362 hasAuthorship W2021213362A5033772999 @default.
• W2021213362 hasAuthorship W2021213362A5046727928 @default.
• W2021213362 hasAuthorship W2021213362A5047966980 @default.
• W2021213362 hasAuthorship W2021213362A5090717064 @default.
• W2021213362 hasBestOaLocation W20212133621 @default.
• W2021213362 hasConcept C104317684 @default.
• W2021213362 hasConcept C141231307 @default.
• W2021213362 hasConcept C143065580 @default.
• W2021213362 hasConcept C153911025 @default.
• W2021213362 hasConcept C182420341 @default.
• W2021213362 hasConcept C204241405 @default.
• W2021213362 hasConcept C2775988993 @default.
• W2021213362 hasConcept C2779491563 @default.
• W2021213362 hasConcept C54355233 @default.
• W2021213362 hasConcept C552990157 @default.
• W2021213362 hasConcept C86803240 @default.
• W2021213362 hasConcept C95444343 @default.
• W2021213362 hasConceptScore W2021213362C104317684 @default.
• W2021213362 hasConceptScore W2021213362C141231307 @default.
• W2021213362 hasConceptScore W2021213362C143065580 @default.
• W2021213362 hasConceptScore W2021213362C153911025 @default.
• W2021213362 hasConceptScore W2021213362C182420341 @default.
• W2021213362 hasConceptScore W2021213362C204241405 @default.
• W2021213362 hasConceptScore W2021213362C2775988993 @default.
• W2021213362 hasConceptScore W2021213362C2779491563 @default.
• W2021213362 hasConceptScore W2021213362C54355233 @default.
• W2021213362 hasConceptScore W2021213362C552990157 @default.
• W2021213362 hasConceptScore W2021213362C86803240 @default.
• W2021213362 hasConceptScore W2021213362C95444343 @default.
• W2021213362 hasIssue "6" @default.
• W2021213362 hasLocation W20212133621 @default.
• W2021213362 hasLocation W20212133622 @default.
• W2021213362 hasOpenAccess W2021213362 @default.
• W2021213362 hasPrimaryLocation W20212133621 @default.
• W2021213362 hasRelatedWork W1594012662 @default.
• W2021213362 hasRelatedWork W1987749596 @default.

• next