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- W2021304721 abstract "The first leaky auxotrophic mutant for aromatic amino acids of the near-diploid fungal plant pathogen Verticillium longisporum (VL) has been generated. VL enters its host Brassica napus through the roots and colonizes the xylem vessels. The xylem contains little nutrients including low concentrations of amino acids. We isolated the gene Vlaro2 encoding chorismate synthase by complementation of the corresponding yeast mutant strain. Chorismate synthase produces the first branch point intermediate of aromatic amino acid biosynthesis. A novel RNA-mediated gene silencing method reduced gene expression of both isogenes by 80% and resulted in a bradytrophic mutant, which is a leaky auxotroph due to impaired expression of chorismate synthase. In contrast to the wild type, silencing resulted in increased expression of the cross-pathway regulatory gene VlcpcA (similar to cpcA/GCN4) during saprotrophic life. The mutant fungus is still able to infect the host plant B. napus and the model Arabidopsis thaliana with reduced efficiency. VlcpcA expression is increased in planta in the mutant and the wild-type fungus. We assume that xylem colonization requires induction of the cross-pathway control, presumably because the fungus has to overcome imbalanced amino acid supply in the xylem." @default.
- W2021304721 created "2016-06-24" @default.
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- W2021304721 date "2009-10-14" @default.
- W2021304721 modified "2023-10-14" @default.
- W2021304721 title "Silencing of Vlaro2 for chorismate synthase revealed that the phytopathogen Verticillium longisporum induces the cross-pathway control in the xylem" @default.
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- W2021304721 cites W2000135985 @default.
- W2021304721 cites W2004740340 @default.
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- W2021304721 doi "https://doi.org/10.1007/s00253-009-2269-0" @default.
- W2021304721 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2811248" @default.
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