Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021307559> ?p ?o ?g. }
- W2021307559 endingPage "930" @default.
- W2021307559 startingPage "919" @default.
- W2021307559 abstract "Jiang-Zhi-Ning (JZN) is composed of four Chinese herbs, i.e., Fleeceflower Root, Fructus Crataegi, Folium Nelumbinis and Semen Cassiae. It was used to strengthen blood circulation of coronary artery, arrhythmia and hyperlipidemia.The main objective of this paper is to evaluate lipid-lowering and antioxidant activities of extract and effective fraction of JZN by using in vitro experiments on hyperlipidemic rats. Moreover, in vivo experiments on cells were performed to investigate lipid-lowering and antioxidant activities of effective fraction and active constituents of JZN.Wistar rats with high fat diet-induced hyperlipidemia were used as in vitro models to study biological effects of lipid-lowering and antioxidant activities of extract and effective fraction of JZN. Serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), Coronary Index and Atherogenic Index were investigated to evaluate lipid-lowering effects of extract and effective fraction of JZN. Serum total nitric oxide synthase (NOS), nitric oxide (NO), endothelin-1 (ET-1), malondialdehyde (MDA), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) were detected to measure antioxidant effects of extract and effective fraction of JZN. Furthermore, oxidized low-density lipoprotein (Ox-LDL) injured human umbilical vein endothelial cell (HUVEC) model was employed as in vivo experiment to study lipid-lowering and antioxidant effects of effective fraction and active constituents of JZN. NO, ET-1, MDA SOD and T-AOC in HUVECs or culture media were investigated to evaluate antioxidant activity of effective fraction and active constituents of JZN. Using human hepatoma cell line Bel-7402, reverse transcription polymerase chain reaction (RT-PCR) technology was performed to investigate cholesterol metabolism effects of effective fraction and active constituents of JZN. Expressions of low density lipoprotein receptor (LDL-R), 3-hydroxy-3-methyl-HMG-coenzyme A reductase (HMG-CoAR), and cholesterol 7α-hydroxylase (CYP7A1) mRNA of the liver cells were investigated to evaluate JZN on associated receptor and enzymes of cholesterol metabolism. High-performance liquid chromatography (HPLC) and spectrophotometry were used to study the impact of effective fraction and active constituents of JZN on synthesis and translation of cholesterol during the process of metabolism by measuring inside and extracellular contents of total bile acid (TBA) of Bel-7402.Extract and effective fraction of JZN significantly reduced contents of TC, TG and LDL-C, CRI and AI in hyperlipidemic rats as well as significantly increased contents of HDL-C in the rats. Moreover, they significantly enhanced the activity of NOS and increased contents of NO. They also caused significant reductions in contents of ET-1 and MDA as well as significant increase in SOD activity and T-AOC in the hyperlipidemic rats. Several indicators were found to be concentration-dependent. As far as in vivo experiments to investigate biological activities of effective fraction and active constituents of JZN were concerned, it was found that they restored and enhanced the vitality of HUVECs with a concentration-dependent manner as well as content of NO in the culture media of HUVEC. They caused reductions in the contents of ET-1 in the culture media of HUVEC and contents of MDA in HUVECs. They also caused an increase in the vitality of SOD and T-AOC in HUVECs. Furthermore, they enhanced LDL-RmRNA expression, with a concentration-dependent manner. Low and medium concentrations of effective fraction and active constituents of JZN could inhibit expression of HMG-CoAR mRNA. High concentration counterpart could enhance expression of the HMG-CoAR mRNA. They enhanced expression of CYP7A1 mRNA in a concentration-dependent manner. Finally, they caused reductions in the contents of cholesterol in Bel-7402. They also increased intercellular content of total bile acid as well as lowered extracellular contents of TBA in the cells in a concentration-dependent manner.We demonstrated for the first time lipid-lowering and antioxidant activities of extract and effective fractions as well as active constituents of JZN. Active constituents of JZN had the same biological effects with effective fraction and extract of JZN. Therefore, this study supports its ethnopharmacological use in Traditional Chinese Medicine to manage hyperlipidemia and paves a basis for establishing quality control method of Chinese medicine." @default.
- W2021307559 created "2016-06-24" @default.
- W2021307559 creator A5012615787 @default.
- W2021307559 creator A5014528965 @default.
- W2021307559 creator A5037959654 @default.
- W2021307559 creator A5039593105 @default.
- W2021307559 creator A5046597133 @default.
- W2021307559 creator A5089948952 @default.
- W2021307559 date "2011-04-01" @default.
- W2021307559 modified "2023-10-17" @default.
- W2021307559 title "Lipid-lowering and antioxidant activities of Jiang-Zhi-Ning in Traditional Chinese Medicine" @default.
- W2021307559 cites W1601241121 @default.
- W2021307559 cites W1995032745 @default.
- W2021307559 cites W2015373623 @default.
- W2021307559 cites W2018825286 @default.
- W2021307559 cites W2025782661 @default.
- W2021307559 cites W2028693103 @default.
- W2021307559 cites W2030078522 @default.
- W2021307559 cites W2067236087 @default.
- W2021307559 cites W2082590619 @default.
- W2021307559 cites W2092054388 @default.
- W2021307559 cites W2129750583 @default.
- W2021307559 cites W2132094039 @default.
- W2021307559 cites W2171929751 @default.
- W2021307559 cites W2181346913 @default.
- W2021307559 cites W2303530524 @default.
- W2021307559 doi "https://doi.org/10.1016/j.jep.2011.01.048" @default.
- W2021307559 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21316437" @default.
- W2021307559 hasPublicationYear "2011" @default.
- W2021307559 type Work @default.
- W2021307559 sameAs 2021307559 @default.
- W2021307559 citedByCount "41" @default.
- W2021307559 countsByYear W20213075592012 @default.
- W2021307559 countsByYear W20213075592013 @default.
- W2021307559 countsByYear W20213075592014 @default.
- W2021307559 countsByYear W20213075592015 @default.
- W2021307559 countsByYear W20213075592016 @default.
- W2021307559 countsByYear W20213075592017 @default.
- W2021307559 countsByYear W20213075592018 @default.
- W2021307559 countsByYear W20213075592019 @default.
- W2021307559 countsByYear W20213075592020 @default.
- W2021307559 countsByYear W20213075592021 @default.
- W2021307559 countsByYear W20213075592023 @default.
- W2021307559 crossrefType "journal-article" @default.
- W2021307559 hasAuthorship W2021307559A5012615787 @default.
- W2021307559 hasAuthorship W2021307559A5014528965 @default.
- W2021307559 hasAuthorship W2021307559A5037959654 @default.
- W2021307559 hasAuthorship W2021307559A5039593105 @default.
- W2021307559 hasAuthorship W2021307559A5046597133 @default.
- W2021307559 hasAuthorship W2021307559A5089948952 @default.
- W2021307559 hasConcept C134018914 @default.
- W2021307559 hasConcept C150903083 @default.
- W2021307559 hasConcept C178790620 @default.
- W2021307559 hasConcept C185592680 @default.
- W2021307559 hasConcept C207001950 @default.
- W2021307559 hasConcept C2775838275 @default.
- W2021307559 hasConcept C2778004101 @default.
- W2021307559 hasConcept C2778163477 @default.
- W2021307559 hasConcept C2778401633 @default.
- W2021307559 hasConcept C2778913445 @default.
- W2021307559 hasConcept C2779091943 @default.
- W2021307559 hasConcept C2779620165 @default.
- W2021307559 hasConcept C2780541478 @default.
- W2021307559 hasConcept C519581460 @default.
- W2021307559 hasConcept C55493867 @default.
- W2021307559 hasConcept C555293320 @default.
- W2021307559 hasConcept C556039675 @default.
- W2021307559 hasConcept C71924100 @default.
- W2021307559 hasConcept C86803240 @default.
- W2021307559 hasConcept C98274493 @default.
- W2021307559 hasConceptScore W2021307559C134018914 @default.
- W2021307559 hasConceptScore W2021307559C150903083 @default.
- W2021307559 hasConceptScore W2021307559C178790620 @default.
- W2021307559 hasConceptScore W2021307559C185592680 @default.
- W2021307559 hasConceptScore W2021307559C207001950 @default.
- W2021307559 hasConceptScore W2021307559C2775838275 @default.
- W2021307559 hasConceptScore W2021307559C2778004101 @default.
- W2021307559 hasConceptScore W2021307559C2778163477 @default.
- W2021307559 hasConceptScore W2021307559C2778401633 @default.
- W2021307559 hasConceptScore W2021307559C2778913445 @default.
- W2021307559 hasConceptScore W2021307559C2779091943 @default.
- W2021307559 hasConceptScore W2021307559C2779620165 @default.
- W2021307559 hasConceptScore W2021307559C2780541478 @default.
- W2021307559 hasConceptScore W2021307559C519581460 @default.
- W2021307559 hasConceptScore W2021307559C55493867 @default.
- W2021307559 hasConceptScore W2021307559C555293320 @default.
- W2021307559 hasConceptScore W2021307559C556039675 @default.
- W2021307559 hasConceptScore W2021307559C71924100 @default.
- W2021307559 hasConceptScore W2021307559C86803240 @default.
- W2021307559 hasConceptScore W2021307559C98274493 @default.
- W2021307559 hasFunder F4320321001 @default.
- W2021307559 hasIssue "3" @default.
- W2021307559 hasLocation W20213075591 @default.
- W2021307559 hasLocation W20213075592 @default.
- W2021307559 hasOpenAccess W2021307559 @default.
- W2021307559 hasPrimaryLocation W20213075591 @default.
- W2021307559 hasRelatedWork W2043571658 @default.
- W2021307559 hasRelatedWork W2063798369 @default.