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- W2021336698 abstract "Whole cells of Micrococcus luteus (formerly Sarcina lutea ATCC 9341) have been covalently linked to a carboxymethylcellulose support system, with the retention of histidine ammonia–lyase activity. The dependence of the rate of urocanic acid formation on pH, temperature, and added surfactant concentration was similar for the free and the immobilized cells. The immobilization procedure used is based on the carbodiimide activation of carboxymethylcellulose and has been optimized for the histidine ammonia–lyase activity of the immobilized cells on a given weight of cellulose. In a column reactor at 23°C and superficial velocity of 0.044 cm/min, 5 g of cellulose with bound cells gave a 35% conversion of an L-histidine solution (0.25M, pH 9.0) to urocanic acid for 16 days of continuous operation. The scope of this carbodiimide assisted immobilization procedure has been investigated for a series of microorganisms and a variety of carboxylate functionalized supports." @default.
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- W2021336698 date "1977-05-01" @default.
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- W2021336698 title "The enzymatic conversion ofL-histidine to urocanic acid by whole cells ofMicrococcus luteus immobilized on carbodiimide activated carboxymethylcellulose" @default.
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- W2021336698 doi "https://doi.org/10.1002/bit.260190503" @default.
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