Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021350894> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2021350894 endingPage "626" @default.
- W2021350894 startingPage "622" @default.
- W2021350894 abstract "We report a simple method to directly label or modify a specific terminus of linear DNA molecules. The method is based upon our finding that a presumably triple-stranded structure by RecA-mediated formation at the terminus formed with deoxyoligonucleotides, whose sequence is complementary to the 5′ terminus of one of the strands of a double-stranded DNA molecule, is quite stable and can serve as a template for DNA polymerase reaction, with the nucleotides being incorporated by an exchange reaction. This novel type of nucleotide incorporation has made it possible to label a specific terminus of target double-stranded DNA molecules by a direct means (without amplification) regardless of its molecular size, a procedure previously unavailable. As an application, we show that large DNA molecules can be fixed to a solid support in a specific orientation, thus being utilized for various analytical purposes of DNA molecules." @default.
- W2021350894 created "2016-06-24" @default.
- W2021350894 creator A5023099049 @default.
- W2021350894 creator A5082322403 @default.
- W2021350894 date "2007-05-01" @default.
- W2021350894 modified "2023-09-23" @default.
- W2021350894 title "Specific terminal labeling of DNA molecules" @default.
- W2021350894 cites W1484560547 @default.
- W2021350894 cites W182692292 @default.
- W2021350894 cites W2093129935 @default.
- W2021350894 cites W2132373675 @default.
- W2021350894 cites W2136372718 @default.
- W2021350894 doi "https://doi.org/10.2144/000112418" @default.
- W2021350894 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17515201" @default.
- W2021350894 hasPublicationYear "2007" @default.
- W2021350894 type Work @default.
- W2021350894 sameAs 2021350894 @default.
- W2021350894 citedByCount "1" @default.
- W2021350894 countsByYear W20213508942023 @default.
- W2021350894 crossrefType "journal-article" @default.
- W2021350894 hasAuthorship W2021350894A5023099049 @default.
- W2021350894 hasAuthorship W2021350894A5082322403 @default.
- W2021350894 hasBestOaLocation W20213508941 @default.
- W2021350894 hasConcept C104317684 @default.
- W2021350894 hasConcept C12554922 @default.
- W2021350894 hasConcept C156719811 @default.
- W2021350894 hasConcept C178790620 @default.
- W2021350894 hasConcept C185581394 @default.
- W2021350894 hasConcept C185592680 @default.
- W2021350894 hasConcept C195116880 @default.
- W2021350894 hasConcept C2756471 @default.
- W2021350894 hasConcept C31254050 @default.
- W2021350894 hasConcept C32909587 @default.
- W2021350894 hasConcept C49105822 @default.
- W2021350894 hasConcept C512185932 @default.
- W2021350894 hasConcept C552990157 @default.
- W2021350894 hasConcept C55493867 @default.
- W2021350894 hasConcept C84148353 @default.
- W2021350894 hasConcept C86803240 @default.
- W2021350894 hasConceptScore W2021350894C104317684 @default.
- W2021350894 hasConceptScore W2021350894C12554922 @default.
- W2021350894 hasConceptScore W2021350894C156719811 @default.
- W2021350894 hasConceptScore W2021350894C178790620 @default.
- W2021350894 hasConceptScore W2021350894C185581394 @default.
- W2021350894 hasConceptScore W2021350894C185592680 @default.
- W2021350894 hasConceptScore W2021350894C195116880 @default.
- W2021350894 hasConceptScore W2021350894C2756471 @default.
- W2021350894 hasConceptScore W2021350894C31254050 @default.
- W2021350894 hasConceptScore W2021350894C32909587 @default.
- W2021350894 hasConceptScore W2021350894C49105822 @default.
- W2021350894 hasConceptScore W2021350894C512185932 @default.
- W2021350894 hasConceptScore W2021350894C552990157 @default.
- W2021350894 hasConceptScore W2021350894C55493867 @default.
- W2021350894 hasConceptScore W2021350894C84148353 @default.
- W2021350894 hasConceptScore W2021350894C86803240 @default.
- W2021350894 hasIssue "5" @default.
- W2021350894 hasLocation W20213508941 @default.
- W2021350894 hasLocation W20213508942 @default.
- W2021350894 hasOpenAccess W2021350894 @default.
- W2021350894 hasPrimaryLocation W20213508941 @default.
- W2021350894 hasRelatedWork W1643501146 @default.
- W2021350894 hasRelatedWork W1989399363 @default.
- W2021350894 hasRelatedWork W1989887297 @default.
- W2021350894 hasRelatedWork W1993876688 @default.
- W2021350894 hasRelatedWork W2031946492 @default.
- W2021350894 hasRelatedWork W2062263965 @default.
- W2021350894 hasRelatedWork W2087714034 @default.
- W2021350894 hasRelatedWork W2089017439 @default.
- W2021350894 hasRelatedWork W2313395946 @default.
- W2021350894 hasRelatedWork W2462629497 @default.
- W2021350894 hasVolume "42" @default.
- W2021350894 isParatext "false" @default.
- W2021350894 isRetracted "false" @default.
- W2021350894 magId "2021350894" @default.
- W2021350894 workType "article" @default.