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• W2021398493 abstract "A Co2+-affinity, fast-flow perfusion chromatography method to purify a polyhistidine-tagged myoglobin-like aerotaxis transducer HemAT-Hs has been developed. The method relies upon a six-histidine affinity tag fused to the C-terminus and N-terminus of HemAT-Hs for expression in the native host, an extremely halophilic Archaeon Halobacterium salinarum, and in the heterologous host Escherichia coli, respectively. The His-tagged HemAT-Hs can be purified rapidly using either low or high ionic strength buffers. Purified His-tagged HemAT-Hs in high or low salt buffers demonstrated no difference in spectral characteristics and retained reversible oxygen binding capacity. This fast-flow Co2+-affinity perfusion chromatography provides a simple method for preparation of halophilic heme containing soluble proteins for biophysical and structural studies." @default.
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• W2021398493 date "2000-12-01" @default.
• W2021398493 modified "2023-10-13" @default.
• W2021398493 title "Expression and fast-flow purification of a polyhistidine-tagged myoglobin-like aerotaxis transducer" @default.
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• W2021398493 doi "https://doi.org/10.1016/s0304-4165(00)00151-3" @default.
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