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- W2021426504 abstract "Vaccinia viral polypeptides, labeled in the presence of rifampicin, were incorporated into virus particles after rifampicin was removed. All structural polypeptides, resolved by polyacrylamide gel electrophoresis, were labeled in the presence of rifampicin. The specific effect of rifampicin on the formation of the vaccinia viral envelope was reversed within 10 min even in the presence of NaF or inhibitors of protein or nucleic acid synthesis. Further maturation of some virus particles, which were characterized by their ultrastructure and isolated by sucrose gradient sedimentation, proceeded in the presence of inhibitors of protein synthesis. In contrast, addition of NaF or actinomycin D almost completely blocked the development of mature virus particles. Rifampicin acted specifically at the stage of envelope formation and did not prevent later steps in maturation." @default.
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- W2021426504 date "1971-07-01" @default.
- W2021426504 modified "2023-10-14" @default.
- W2021426504 title "Assembly of vaccinia virus particles from polypeptides made in the presence of rifampicin" @default.
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- W2021426504 doi "https://doi.org/10.1016/0042-6822(71)90119-x" @default.
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