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- W2021455571 abstract "A method has been developed for the isolation of RNA from E. coli “protoplasts”, by extraction with a phenol-water mixture. The RNA preparation contained no detectable DNA and only traces of proteins and polysaccharides. Sedimentation in the ultracentrifuge yielded three boundaries. The two faster moving components were separated from the slower one by (NH4)2SO4 precipitation from a phenol-saturated water solution. From sedimentation, viscosity and light-scattering data the molecular weight was estimated to be of the order of one million. The RNA preparations in their viscosity behavior closely resemble coiling synthetic polyelectrolytes, and behave quite unlike DNA. The viscosity behavior, birefringence of flow, and potentiometric titration data are discussed in terms of a single contractile coil model. The ϵ(P)260 mμ values of the isolated material in 1 M phosphate (pH 7.1) were about 7400. Alkaline hydrolysis or polyribonucleotide phosphorylase action brought about an increase in absorption ranging from 56 to 59% while pancreatic ribonuclease caused a 28–31% increment. At very low concentrations of RNAase the change in optical density remained constant, while viscosity decreased rapidly with time." @default.
- W2021455571 created "2016-06-24" @default.
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- W2021455571 date "1959-01-01" @default.
- W2021455571 modified "2023-10-13" @default.
- W2021455571 title "Ribonucleic acid from Escherichia coli preparation, characterization and physical properties" @default.
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- W2021455571 doi "https://doi.org/10.1016/0006-3002(59)90604-3" @default.
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