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• W2021489809 abstract "Abstract We have constructed plasmids carrying d(AT)n·d(AT)n inserts of different lengths. Two- dimensional gel electrophoresis patterns show that an increase in the negative superhelicity of these DNAs brings about a structural transition within the inserts, resulting in a reduction of the superhelical stress. However, this reduction corresponds to the expected values neither for cruciform nor for the Z form. Those DNA topoisomers in which the structural transition had occurred proved to be specifically recognizable by single-strand-specific endonuclease SI, with the cleavage site situated at the centre of the insert. These data, as well as kinetic studies, suggest that the cloned d(AT)n·d(AT) n sequences adopt a cruciform rather than the Z-form structure. We discuss plausible reasons of the discrepancy between the observed superhelical stress release and that expected for the transition of the insert to the cruciform state." @default.
• W2021489809 created "2016-06-24" @default.
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• W2021489809 creator A5053934339 @default.
• W2021489809 date "1985-06-01" @default.
• W2021489809 modified "2023-09-27" @default.
• W2021489809 title "A Structural Transition in d(AT)_<i>n</i>·d(AT)_<i>n</i>Inserts within Superhelical DNA" @default.
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• W2021489809 doi "https://doi.org/10.1080/07391102.1985.10507634" @default.
• W2021489809 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3917182" @default.
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