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• W2021504120 abstract "The origin of platelet-factor-V has long been discussed. To elucidate whether and when human platelet-factor-V is synthesized by megakaryocytes, we utilized in vitro-generated megakaryocytes capable of producing platelets. Factor-V gene was silent in purified progenitors and megakaryocytic precursors but was expressed in late culture phase and maintained also in platelets. Similarly, factor-V protein was expressed in mature proplatelet-bearing megakaryocytes (immunofluorescence analysis); it was also detectable in cultured megakaryocytes and platelets (Western blotting) and within permeabilized cultured platelets (flow cytometry). The absence of other cells in our culture system indicates conclusively that human megakaryocytes synthesize factor-V." @default.
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• W2021504120 date "2004-12-07" @default.
• W2021504120 modified "2023-10-17" @default.
• W2021504120 title "Factor-V expression in platelets from human megakaryocytic culture" @default.
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• W2021504120 doi "https://doi.org/10.1111/j.1365-2141.2004.05279.x" @default.
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