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- W2021509788 abstract "BACKGROUND: To contribute towards understanding the relationship of structure and bioactivity, a protein-bound acidic polysaccharide named TPC3-1 was isolated and purified from low-grade green tea (Camellia sinensis L.). The homogeneity and weight average molecular weight of TPC3-1 was determined by agarose gel electrophoresis and high-performance gel permeation chromatography. The monosaccharide and amino acid composition of TPC3-1 were analysed by gas chromatography and an amino acid analyser. The molecular structure of TPC3-1 was characterised by Fourier transform infrared spectroscopy, 13C nuclear magnetic resonance spectroscopy and atomic force microscopy. RESULTS: Based on the data obtained, the average peak molecular weight of TPC3-1 was about 120 kDa. TPC3-1 was composed of L-arabinose, D-ribose, D-xylose, D-glucose and D-galactose with a molar ratio of 4.9:2.2:3.1:1.8:1.0. Fifteen amino acids were identified as components of the polymer. The TPC3-1 molecule was found to have an anomeric carbon sign of both α and β configurations and high-branched chains. The network structure of TPC3-1 was observed. CONCLUSION: The tea polysaccharide TPC3-1 was an acid protein-bound polysaccharide with an image of network structure. The results presented here will facilitate further study of the relationship between the chemical structure and biological role of tea polysaccharide. Copyright © 2008 Society of Chemical Industry" @default.
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- W2021509788 date "2008-11-01" @default.
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- W2021509788 title "Isolation and chemical characterisation of a polysaccharide from green tea (<i>Camellia sinensis</i>L.)" @default.
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- W2021509788 doi "https://doi.org/10.1002/jsfa.3375" @default.
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