Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021516345> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2021516345 endingPage "223" @default.
- W2021516345 startingPage "215" @default.
- W2021516345 abstract "Cellulase-producing fungi from the Andean regions in Bolivia, an ecosystem characterized as an extreme arid highland, were studied. Thirty-two isolates were screened for presence of cellulase activity using carboxymethyl cellulose (CMC) as carbon source, and activity was confirmed using a filter paper assay. One isolate, denoted as BLT1C was selected from this screening, and sequence analysis of the internal transcribed spacer (ITS) classified the strain as Hypocrea lixii. The secretome of BLT1C showed high xylanase activity (compared to that of two reference Trichoderma reesei strains) when cultivated using brave straw, an abundant native grass from the area, as carbon source. SDS-PAGE analysis revealed three main protein-bands (18, 32 and 65 kDa) and in-gel digestion and mass spectrometry combined with activity analysis showed that these proteins were active xylanases with molecular masses corresponding to (I) a single glycoside hydrolase family 11 catalytic module (18 kDa), and (II, III) modular enzymes, with the GH11 catalytic domain connected to a module of unknown function (32 kDa) or putatively connected to a GH7 catalytic module (65 kDa). The N-terminal sequence of the 65 kDa xylanase did not show significant sequence similarities to deposited sequences. The collected data on xylanase activity, molecular mass, GH11-sequence conservation, combined with lack of sequence similarities in the N-terminus show that the 65 kDa band corresponds to a novel modular xylanase." @default.
- W2021516345 created "2016-06-24" @default.
- W2021516345 creator A5002685278 @default.
- W2021516345 creator A5025431061 @default.
- W2021516345 creator A5033042329 @default.
- W2021516345 creator A5037397129 @default.
- W2021516345 date "2012-07-01" @default.
- W2021516345 modified "2023-09-27" @default.
- W2021516345 title "A cellulolytic Hypocrea strain isolated from South American brave straw produces a modular xylanase" @default.
- W2021516345 cites W1968984997 @default.
- W2021516345 cites W1976127558 @default.
- W2021516345 cites W1979048217 @default.
- W2021516345 cites W1988964784 @default.
- W2021516345 cites W1991193571 @default.
- W2021516345 cites W1996873149 @default.
- W2021516345 cites W2007447394 @default.
- W2021516345 cites W2011213012 @default.
- W2021516345 cites W2029216037 @default.
- W2021516345 cites W2038102744 @default.
- W2021516345 cites W2054172648 @default.
- W2021516345 cites W2081347422 @default.
- W2021516345 cites W2099285587 @default.
- W2021516345 cites W2100837269 @default.
- W2021516345 cites W2102530672 @default.
- W2021516345 cites W2112723141 @default.
- W2021516345 cites W2124233578 @default.
- W2021516345 cites W2129940534 @default.
- W2021516345 cites W2148041910 @default.
- W2021516345 cites W2152756549 @default.
- W2021516345 cites W2161849016 @default.
- W2021516345 cites W2169762780 @default.
- W2021516345 cites W4232580600 @default.
- W2021516345 doi "https://doi.org/10.1016/j.carres.2012.03.030" @default.
- W2021516345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22609439" @default.
- W2021516345 hasPublicationYear "2012" @default.
- W2021516345 type Work @default.
- W2021516345 sameAs 2021516345 @default.
- W2021516345 citedByCount "7" @default.
- W2021516345 countsByYear W20215163452013 @default.
- W2021516345 countsByYear W20215163452014 @default.
- W2021516345 countsByYear W20215163452019 @default.
- W2021516345 countsByYear W20215163452023 @default.
- W2021516345 crossrefType "journal-article" @default.
- W2021516345 hasAuthorship W2021516345A5002685278 @default.
- W2021516345 hasAuthorship W2021516345A5025431061 @default.
- W2021516345 hasAuthorship W2021516345A5033042329 @default.
- W2021516345 hasAuthorship W2021516345A5037397129 @default.
- W2021516345 hasConcept C104317684 @default.
- W2021516345 hasConcept C116174643 @default.
- W2021516345 hasConcept C141603559 @default.
- W2021516345 hasConcept C181199279 @default.
- W2021516345 hasConcept C185592680 @default.
- W2021516345 hasConcept C196032511 @default.
- W2021516345 hasConcept C2775859485 @default.
- W2021516345 hasConcept C2776125036 @default.
- W2021516345 hasConcept C2778657544 @default.
- W2021516345 hasConcept C55493867 @default.
- W2021516345 hasConcept C58121356 @default.
- W2021516345 hasConcept C61053724 @default.
- W2021516345 hasConceptScore W2021516345C104317684 @default.
- W2021516345 hasConceptScore W2021516345C116174643 @default.
- W2021516345 hasConceptScore W2021516345C141603559 @default.
- W2021516345 hasConceptScore W2021516345C181199279 @default.
- W2021516345 hasConceptScore W2021516345C185592680 @default.
- W2021516345 hasConceptScore W2021516345C196032511 @default.
- W2021516345 hasConceptScore W2021516345C2775859485 @default.
- W2021516345 hasConceptScore W2021516345C2776125036 @default.
- W2021516345 hasConceptScore W2021516345C2778657544 @default.
- W2021516345 hasConceptScore W2021516345C55493867 @default.
- W2021516345 hasConceptScore W2021516345C58121356 @default.
- W2021516345 hasConceptScore W2021516345C61053724 @default.
- W2021516345 hasLocation W20215163451 @default.
- W2021516345 hasLocation W20215163452 @default.
- W2021516345 hasOpenAccess W2021516345 @default.
- W2021516345 hasPrimaryLocation W20215163451 @default.
- W2021516345 hasRelatedWork W1903069539 @default.
- W2021516345 hasRelatedWork W1984169702 @default.
- W2021516345 hasRelatedWork W1991209722 @default.
- W2021516345 hasRelatedWork W2021516345 @default.
- W2021516345 hasRelatedWork W2055897495 @default.
- W2021516345 hasRelatedWork W2071798435 @default.
- W2021516345 hasRelatedWork W2803514297 @default.
- W2021516345 hasRelatedWork W2941978624 @default.
- W2021516345 hasRelatedWork W3003919054 @default.
- W2021516345 hasRelatedWork W3015690328 @default.
- W2021516345 hasVolume "356" @default.
- W2021516345 isParatext "false" @default.
- W2021516345 isRetracted "false" @default.
- W2021516345 magId "2021516345" @default.
- W2021516345 workType "article" @default.