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- W2021523748 abstract "A microtiter plate was coated with cell nuclei from HEp-2 cells, and the stabilized nuclei were fixed with acetone for an enzyme-linked immunosorbent assay (ELISA). Auto-antibodies against nuclear antigens were detected from the sera of patients with various systemic rheumatic disease but not from healthy individuals by means of the nucleus ELISA procedure. Ninety-one percent of anti-nuclear antibody (ANA)—positive sera as demonstrated by immunofluorescence (IF) test were also judged as positive for ANAs by the nucleus ELISA and 5% of them as psudo-positive. Patient's sera with homogeneous and fine-speckled IF patterns displayed the highest ELISA titers. A large portion of ss-A/Ro antigen is localized in the cytoplasm; therefore, anti-ss-A/Ro antibody was hardly detected by nucleus ELISA. The population of ANAs detectable by nucleus ELISA included anti-ss-B/La, anti-DNA, anti-histone, anti-Sm, anti-RNP, and anti-scl-70." @default.
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- W2021523748 date "1991-01-01" @default.
- W2021523748 modified "2023-09-25" @default.
- W2021523748 title "Detection of anti-nuclear antibodies from patients with systemic rheumatic diseases by elisa using hep-2 cell nuclei" @default.
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- W2021523748 doi "https://doi.org/10.1002/jcla.1860050109" @default.
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