FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021531373> ?p ?o ?g. }

• W2021531373 endingPage "685" @default.
• W2021531373 startingPage "678" @default.
• W2021531373 abstract "The use of cheap and thermoresistant vaccines in poor tropical countries for the control of animal diseases is a key issue. Our work aimed at designing and validating a process for the large-scale production of a ready-to-use inactivated vaccine for ruminants. Our model was heartwater caused by the obligate intracellular bacterium Ehrlichia ruminantium (ER). The conventional inactivated vaccine against heartwater (based on whole bacteria inactivated with sodium azide) is prepared immediately before injection, using a syringe-extrusion method with Montanide ISA50. This is a fastidious time-consuming process and it limits the number of vaccine doses available. To overcome these issues, we tested three different techniques (syringe, vortex and homogenizer) and three Montanide ISA adjuvants (50, 70 and 70M). High-speed homogenizer was the optimal method to emulsify ER antigens with both ISA70 and 70M adjuvants. The emulsions displayed a good homogeneity (particle size below 1 μm and low phase separation), conductivity below 10 μS/cm and low antigen degradation at 4 °C for up to 1 year. The efficacy of the different formulations was then evaluated during vaccination trials on goats. The inactivated ER antigens emulsified with ISA70 and ISA70M in a homogenizer resulted in 80% and 100% survival rates, respectively. A cold-chain rupture assay using ISA70M+ER was performed to mimic possible field conditions exposing the vaccine at 37 °C for 4 days before delivery. Surprisingly, the animal survival rate was still high (80%). We also observed that the MAP-1B antibody response was very similar between animals vaccinated with ISA70+ER and ISA70M+ER emulsions, suggesting a more homogenous antigen distribution and presentation in these emulsions. Our work demonstrated that the combination of ISA70 or ISA70M and homogenizer is optimal for the production of an effective ready-to-use inactivated vaccine against heartwater, which could easily be produced on an industrial scale." @default.
• W2021531373 created "2016-06-24" @default.
• W2021531373 creator A5005593666 @default.
• W2021531373 creator A5016427165 @default.
• W2021531373 creator A5034877650 @default.
• W2021531373 creator A5051978915 @default.
• W2021531373 creator A5054955704 @default.
• W2021531373 creator A5060764244 @default.
• W2021531373 creator A5060771518 @default.
• W2021531373 creator A5070704686 @default.
• W2021531373 creator A5076998761 @default.
• W2021531373 date "2015-01-01" @default.
• W2021531373 modified "2023-09-26" @default.
• W2021531373 title "A user-friendly and scalable process to prepare a ready-to-use inactivated vaccine: The example of heartwater in ruminants under tropical conditions" @default.
• W2021531373 cites W1970433063 @default.
• W2021531373 cites W1979127206 @default.
• W2021531373 cites W2001745898 @default.
• W2021531373 cites W2005925031 @default.
• W2021531373 cites W2006783355 @default.
• W2021531373 cites W2008100229 @default.
• W2021531373 cites W2014817731 @default.
• W2021531373 cites W2017503466 @default.
• W2021531373 cites W2023179911 @default.
• W2021531373 cites W2028597344 @default.
• W2021531373 cites W2032426044 @default.
• W2021531373 cites W2038088213 @default.
• W2021531373 cites W2039237789 @default.
• W2021531373 cites W2051566491 @default.
• W2021531373 cites W2065519739 @default.
• W2021531373 cites W2073256556 @default.
• W2021531373 cites W2078011555 @default.
• W2021531373 cites W2080983733 @default.
• W2021531373 cites W2087709764 @default.
• W2021531373 cites W2087961202 @default.
• W2021531373 cites W2099183534 @default.
• W2021531373 cites W2103084045 @default.
• W2021531373 cites W2114418581 @default.
• W2021531373 cites W2126650457 @default.
• W2021531373 cites W2126985251 @default.
• W2021531373 cites W2152381499 @default.
• W2021531373 cites W2162795747 @default.
• W2021531373 cites W2169255619 @default.
• W2021531373 doi "https://doi.org/10.1016/j.vaccine.2014.11.059" @default.
• W2021531373 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25514207" @default.
• W2021531373 hasPublicationYear "2015" @default.
• W2021531373 type Work @default.
• W2021531373 sameAs 2021531373 @default.
• W2021531373 citedByCount "5" @default.
• W2021531373 countsByYear W20215313732015 @default.
• W2021531373 countsByYear W20215313732016 @default.
• W2021531373 countsByYear W20215313732017 @default.
• W2021531373 countsByYear W20215313732021 @default.
• W2021531373 crossrefType "journal-article" @default.
• W2021531373 hasAuthorship W2021531373A5005593666 @default.
• W2021531373 hasAuthorship W2021531373A5016427165 @default.
• W2021531373 hasAuthorship W2021531373A5034877650 @default.
• W2021531373 hasAuthorship W2021531373A5051978915 @default.
• W2021531373 hasAuthorship W2021531373A5054955704 @default.
• W2021531373 hasAuthorship W2021531373A5060764244 @default.
• W2021531373 hasAuthorship W2021531373A5060771518 @default.
• W2021531373 hasAuthorship W2021531373A5070704686 @default.
• W2021531373 hasAuthorship W2021531373A5076998761 @default.
• W2021531373 hasConcept C147483822 @default.
• W2021531373 hasConcept C159047783 @default.
• W2021531373 hasConcept C192797455 @default.
• W2021531373 hasConcept C203014093 @default.
• W2021531373 hasConcept C22070199 @default.
• W2021531373 hasConcept C2776872762 @default.
• W2021531373 hasConcept C42972112 @default.
• W2021531373 hasConcept C54355233 @default.
• W2021531373 hasConcept C71924100 @default.
• W2021531373 hasConcept C86803240 @default.
• W2021531373 hasConcept C89423630 @default.
• W2021531373 hasConceptScore W2021531373C147483822 @default.
• W2021531373 hasConceptScore W2021531373C159047783 @default.
• W2021531373 hasConceptScore W2021531373C192797455 @default.
• W2021531373 hasConceptScore W2021531373C203014093 @default.
• W2021531373 hasConceptScore W2021531373C22070199 @default.
• W2021531373 hasConceptScore W2021531373C2776872762 @default.
• W2021531373 hasConceptScore W2021531373C42972112 @default.
• W2021531373 hasConceptScore W2021531373C54355233 @default.
• W2021531373 hasConceptScore W2021531373C71924100 @default.
• W2021531373 hasConceptScore W2021531373C86803240 @default.
• W2021531373 hasConceptScore W2021531373C89423630 @default.
• W2021531373 hasIssue "5" @default.
• W2021531373 hasLocation W20215313731 @default.
• W2021531373 hasLocation W20215313732 @default.
• W2021531373 hasOpenAccess W2021531373 @default.
• W2021531373 hasPrimaryLocation W20215313731 @default.
• W2021531373 hasRelatedWork W1487857142 @default.
• W2021531373 hasRelatedWork W1702322095 @default.
• W2021531373 hasRelatedWork W1865764402 @default.
• W2021531373 hasRelatedWork W1975114493 @default.
• W2021531373 hasRelatedWork W2004452559 @default.
• W2021531373 hasRelatedWork W2056557014 @default.
• W2021531373 hasRelatedWork W2065651662 @default.
• W2021531373 hasRelatedWork W2889659110 @default.
• W2021531373 hasRelatedWork W4311522585 @default.

• next