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- W2021532467 abstract "A simple and rapid solid-phase reverse transcriptase assay was developed based on the use of poly(rA):oligo(dT)12–18 as template primer immobilized on DEAE cellulose paper squares to detect human immunodeficiency virus (HIV) and/or Other retroviruses in cell culture supernatants. It was found that PEG (per se) — up to 4% concentrations (w/v) — did not inhibit reverse transcriptase activity. Optimal conditions of the assay were determined. This solid-phase technique is much faster and more convenient than the methods described previously." @default.
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- W2021532467 date "1990-03-01" @default.
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- W2021532467 title "A solid phase reverse transcriptase micro-assay for the detection of human immunodeficiency virus and other retroviruses in cell culture supernatants" @default.
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- W2021532467 doi "https://doi.org/10.1016/0166-0934(90)90095-w" @default.
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