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- W2021541908 abstract "We have generated a novel model protein for an artificial light-harvesting complex composed of two proteins, cytochrome b(562) (cytb(562)) and enhanced green fluorescent protein (EGFP), in which two chromophores are fixed in each protein matrix. Cytb(562) was appended to the N-terminus of EGFP via a Gly-Ser linker and the resultant fusion protein was successfully expressed in Escherichia coli as a mixture of the apo- and the holo-forms as to the cytb(562) moiety. The fluorescence of EGFP was substantially quenched when the apo-form was reconstituted with hemin. Based on the fluorescence lifetime measurements, it appeared that light energy entrapped by EGFP is transferred to the heme of cytb(562) by resonance energy transfer (energy transfer yield: 65%). Spatial organization of two chromophores using small and stable protein matrices will be promising toward the construction of an artificial light-harvesting complex by protein engineering." @default.
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- W2021541908 date "2001-11-01" @default.
- W2021541908 modified "2023-09-30" @default.
- W2021541908 title "Design of an Artificial Light-Harvesting Unit by Protein Engineering: Cytochrome b562–Green Fluorescent Protein Chimera" @default.
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- W2021541908 doi "https://doi.org/10.1006/bbrc.2001.5966" @default.
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