FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021569883> ?p ?o ?g. }

• W2021569883 endingPage "396" @default.
• W2021569883 startingPage "383" @default.
• W2021569883 abstract "Lung vascular alterations and pulmonary hypertension associated with oxidative stress have been reported to be involved in idiopathic lung fibrosis (ILF). Therefore, here, we hypothesize that the widely used lung fibrosis inducer, bleomycin, would cause cytoskeletal rearrangement through thiol-redox alterations in the cultured lung vascular endothelial cell (EC) monolayers. We exposed the monolayers of primary bovine pulmonary artery ECs to bleomycin (10 µg) and studied the cytotoxicity, cytoskeletal rearrangements, and the macromolecule (fluorescein isothiocyanate-dextran, 70,000 mol. wt.) paracellular transport in the absence and presence of two thiol-redox protectants, the classic water-soluble N-acetyl-l-cysteine (NAC) and the novel hydrophobic N,N′-bis-2-mercaptoethyl isophthalamide (NBMI). Our results revealed that bleomycin induced cytotoxicity (lactate dehydrogenase leak), morphological alterations (rounding of cells and filipodia formation), and cytoskeletal rearrangement (actin stress fiber formation and alterations of tight junction proteins, ZO-1 and occludin) in a dose-dependent fashion. Furthermore, our study demonstrated the formation of reactive oxygen species, loss of thiols (glutathione, GSH), EC barrier dysfunction (decrease of transendothelial electrical resistance), and enhanced paracellular transport (leak) of macromolecules. The observed bleomycin-induced EC alterations were attenuated by both NAC and NBMI, revealing that the novel hydrophobic thiol-protectant, NBMI, was more effective at µM concentrations as compared to the water-soluble NAC that was effective at mM concentrations in offering protection against the bleomycin-induced EC alterations. Overall, the results of the current study suggested the central role of thiol-redox in vascular EC dysfunction associated with ILF." @default.
• W2021569883 created "2016-06-24" @default.
• W2021569883 creator A5001932702 @default.
• W2021569883 creator A5004454102 @default.
• W2021569883 creator A5012485816 @default.
• W2021569883 creator A5014085480 @default.
• W2021569883 creator A5016422334 @default.
• W2021569883 creator A5026983357 @default.
• W2021569883 creator A5029971954 @default.
• W2021569883 creator A5035342575 @default.
• W2021569883 creator A5036510967 @default.
• W2021569883 creator A5043003412 @default.
• W2021569883 creator A5022306201 @default.
• W2021569883 date "2012-03-13" @default.
• W2021569883 modified "2023-10-16" @default.
• W2021569883 title "Thiol-redox antioxidants protect against lung vascular endothelial cytoskeletal alterations caused by pulmonary fibrosis inducer, bleomycin: comparison between classical thiol-protectant,<i>N</i>-acetyl-l-cysteine, and novel thiol antioxidant,<i>N,N′</i>-bis-2-mercaptoethyl isophthalamide" @default.
• W2021569883 cites W1516081887 @default.
• W2021569883 cites W1703021606 @default.
• W2021569883 cites W1786137152 @default.
• W2021569883 cites W1969448383 @default.
• W2021569883 cites W1972038951 @default.
• W2021569883 cites W1979629832 @default.
• W2021569883 cites W1981429187 @default.
• W2021569883 cites W1989971071 @default.
• W2021569883 cites W1994839187 @default.
• W2021569883 cites W2007762873 @default.
• W2021569883 cites W2014187911 @default.
• W2021569883 cites W2019121101 @default.
• W2021569883 cites W2026187747 @default.
• W2021569883 cites W2030093453 @default.
• W2021569883 cites W2039570259 @default.
• W2021569883 cites W2040092015 @default.
• W2021569883 cites W2046209146 @default.
• W2021569883 cites W2046501504 @default.
• W2021569883 cites W2047002154 @default.
• W2021569883 cites W2050539799 @default.
• W2021569883 cites W2051556121 @default.
• W2021569883 cites W2052259257 @default.
• W2021569883 cites W2056078569 @default.
• W2021569883 cites W2057602456 @default.
• W2021569883 cites W2061079908 @default.
• W2021569883 cites W2063195524 @default.
• W2021569883 cites W2064307892 @default.
• W2021569883 cites W2066714582 @default.
• W2021569883 cites W2074069122 @default.
• W2021569883 cites W2075707324 @default.
• W2021569883 cites W2076184158 @default.
• W2021569883 cites W2088821423 @default.
• W2021569883 cites W2088889267 @default.
• W2021569883 cites W2090276470 @default.
• W2021569883 cites W2091355057 @default.
• W2021569883 cites W2095464106 @default.
• W2021569883 cites W2097256289 @default.
• W2021569883 cites W2098562755 @default.
• W2021569883 cites W2100801094 @default.
• W2021569883 cites W2111937069 @default.
• W2021569883 cites W2120997345 @default.
• W2021569883 cites W2126965678 @default.
• W2021569883 cites W2129852694 @default.
• W2021569883 cites W2134328303 @default.
• W2021569883 cites W2142457192 @default.
• W2021569883 cites W2147156629 @default.
• W2021569883 cites W2153624387 @default.
• W2021569883 cites W2164155367 @default.
• W2021569883 cites W2167211373 @default.
• W2021569883 cites W2172210768 @default.
• W2021569883 cites W4251243952 @default.
• W2021569883 doi "https://doi.org/10.3109/15376516.2012.673089" @default.
• W2021569883 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3914546" @default.
• W2021569883 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22409285" @default.
• W2021569883 hasPublicationYear "2012" @default.
• W2021569883 type Work @default.
• W2021569883 sameAs 2021569883 @default.
• W2021569883 citedByCount "20" @default.
• W2021569883 countsByYear W20215698832013 @default.
• W2021569883 countsByYear W20215698832014 @default.
• W2021569883 countsByYear W20215698832015 @default.
• W2021569883 countsByYear W20215698832016 @default.
• W2021569883 countsByYear W20215698832017 @default.
• W2021569883 countsByYear W20215698832018 @default.
• W2021569883 countsByYear W20215698832020 @default.
• W2021569883 countsByYear W20215698832021 @default.
• W2021569883 countsByYear W20215698832022 @default.
• W2021569883 crossrefType "journal-article" @default.
• W2021569883 hasAuthorship W2021569883A5001932702 @default.
• W2021569883 hasAuthorship W2021569883A5004454102 @default.
• W2021569883 hasAuthorship W2021569883A5012485816 @default.
• W2021569883 hasAuthorship W2021569883A5014085480 @default.
• W2021569883 hasAuthorship W2021569883A5016422334 @default.
• W2021569883 hasAuthorship W2021569883A5022306201 @default.
• W2021569883 hasAuthorship W2021569883A5026983357 @default.
• W2021569883 hasAuthorship W2021569883A5029971954 @default.
• W2021569883 hasAuthorship W2021569883A5035342575 @default.
• W2021569883 hasAuthorship W2021569883A5036510967 @default.
• W2021569883 hasAuthorship W2021569883A5043003412 @default.
• W2021569883 hasBestOaLocation W20215698832 @default.
• W2021569883 hasConcept C126322002 @default.
• W2021569883 hasConcept C181199279 @default.

• next