Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021570983> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2021570983 endingPage "278" @default.
- W2021570983 startingPage "271" @default.
- W2021570983 abstract "Two enone reductases catalyzing irreversibly the enantioselective reduction of alpha,beta-unsaturated carbonyls have been purified 165-fold and 257-fold, respectively, from the cytosolic fraction of Saccharomyces cerevisiae by means of streptomycin sulfate treatment, Sephadex G-25 filtration, DEAE-Sepharose CL-6B chromatography, blue Sepharose CL-6B chromatography and Superdex 200 preparation-grade filtration. One enzyme (E I) was NADPH-dependent, showed a molecular mass of 75 kDa and decomposed under denaturing electrophoretic conditions into two subunits of 34 kDa and 37 kDa. The other enzyme (E II) was NADH linked and the molecular mass estimated by means of Superdex 200 preparation-grade filtration, was 130 kDa. The enzyme decomposed into subunits of 56 kDa and 64 kDa on SDS/PAGE. Both enzymes were most active at pH 4.8 and accepted 1-octen-3-one, 1-hexen-3-one, 3-alken-2-ones, 4-alken-3-ones, 2-cyclohexen-1-ones, 2-alkenals, 2,4-alkadienals, 2-phenyl-2-alkenals, and 2-alkyl-2-alkenals as substrates. Both enzymes showed their highest activities with 1-hexen-3-one and 1-octen-3-one, respectively. The Km values for 1-octen-3-one were estimated as 0.54 mM (E I) and 0.20 mM (E II), respectively. Both enzymes catalyzed the enantioselective reduction of cis- and trans-2-phenyl-2-butenal into (R)-2-phenylbutanal." @default.
- W2021570983 created "2016-06-24" @default.
- W2021570983 creator A5026858978 @default.
- W2021570983 creator A5070026878 @default.
- W2021570983 date "1998-07-01" @default.
- W2021570983 modified "2023-10-14" @default.
- W2021570983 title "Purification and characterization of two enone reductases from Saccharomyces cerevisiae" @default.
- W2021570983 doi "https://doi.org/10.1046/j.1432-1327.1998.2550271.x" @default.
- W2021570983 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9692928" @default.
- W2021570983 hasPublicationYear "1998" @default.
- W2021570983 type Work @default.
- W2021570983 sameAs 2021570983 @default.
- W2021570983 citedByCount "32" @default.
- W2021570983 countsByYear W20215709832012 @default.
- W2021570983 countsByYear W20215709832014 @default.
- W2021570983 countsByYear W20215709832015 @default.
- W2021570983 countsByYear W20215709832017 @default.
- W2021570983 countsByYear W20215709832020 @default.
- W2021570983 countsByYear W20215709832021 @default.
- W2021570983 countsByYear W20215709832022 @default.
- W2021570983 countsByYear W20215709832023 @default.
- W2021570983 crossrefType "journal-article" @default.
- W2021570983 hasAuthorship W2021570983A5026858978 @default.
- W2021570983 hasAuthorship W2021570983A5070026878 @default.
- W2021570983 hasBestOaLocation W20215709831 @default.
- W2021570983 hasConcept C146686406 @default.
- W2021570983 hasConcept C161790260 @default.
- W2021570983 hasConcept C163994747 @default.
- W2021570983 hasConcept C181199279 @default.
- W2021570983 hasConcept C185592680 @default.
- W2021570983 hasConcept C193363816 @default.
- W2021570983 hasConcept C2777576037 @default.
- W2021570983 hasConcept C2779222958 @default.
- W2021570983 hasConcept C2779881004 @default.
- W2021570983 hasConcept C43617362 @default.
- W2021570983 hasConcept C55493867 @default.
- W2021570983 hasConcept C58121356 @default.
- W2021570983 hasConcept C71240020 @default.
- W2021570983 hasConceptScore W2021570983C146686406 @default.
- W2021570983 hasConceptScore W2021570983C161790260 @default.
- W2021570983 hasConceptScore W2021570983C163994747 @default.
- W2021570983 hasConceptScore W2021570983C181199279 @default.
- W2021570983 hasConceptScore W2021570983C185592680 @default.
- W2021570983 hasConceptScore W2021570983C193363816 @default.
- W2021570983 hasConceptScore W2021570983C2777576037 @default.
- W2021570983 hasConceptScore W2021570983C2779222958 @default.
- W2021570983 hasConceptScore W2021570983C2779881004 @default.
- W2021570983 hasConceptScore W2021570983C43617362 @default.
- W2021570983 hasConceptScore W2021570983C55493867 @default.
- W2021570983 hasConceptScore W2021570983C58121356 @default.
- W2021570983 hasConceptScore W2021570983C71240020 @default.
- W2021570983 hasIssue "1" @default.
- W2021570983 hasLocation W20215709831 @default.
- W2021570983 hasLocation W20215709832 @default.
- W2021570983 hasOpenAccess W2021570983 @default.
- W2021570983 hasPrimaryLocation W20215709831 @default.
- W2021570983 hasRelatedWork W1546532108 @default.
- W2021570983 hasRelatedWork W1579730234 @default.
- W2021570983 hasRelatedWork W1977485891 @default.
- W2021570983 hasRelatedWork W2054158650 @default.
- W2021570983 hasRelatedWork W2085290357 @default.
- W2021570983 hasRelatedWork W2328635228 @default.
- W2021570983 hasRelatedWork W2381180940 @default.
- W2021570983 hasRelatedWork W2384994046 @default.
- W2021570983 hasRelatedWork W4249963955 @default.
- W2021570983 hasRelatedWork W4254878481 @default.
- W2021570983 hasVolume "255" @default.
- W2021570983 isParatext "false" @default.
- W2021570983 isRetracted "false" @default.
- W2021570983 magId "2021570983" @default.
- W2021570983 workType "article" @default.