FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021573022> ?p ?o ?g. }

• W2021573022 abstract "Low positive cell screening efficiency severely hinders the development of transgenic animals. The major rate-limiting step of positive cell screening is DNA entering the nucleus, particularly for large DNA molecules. To enhance the transport of large DNA molecules into the nucleus, particularly for the production of transgenic animals, nuclear localization sequence (NLS) peptides and the peptide derivative succinimidyl-[4-(psoralen-8-yloxy)]-butyrate (SPB)-NLS were synthesized to mediate transfection in vitro. To investigate the function of NLS and SPB-NLS in vitro, the expression levels of growth hormone (GH) mRNA and green fluorescent protein (GFP) protein were analyzed following transfection mediated by NLS and SPB-NLS. The results demonstrated that the expression of GH mRNA was significantly higher in the NLS (increased by 69%) and SPB-NLS (330%) groups than that in the liposome/pGN group. Similarly, GFP expression was found to be higher in the SPB-NLS group than that in the liposome group, while the expression in the NLS group was lower than that in the liposome group. Further analysis demonstrated that SPB-NLS enhanced the expression of insulin-like growth factor 1 in hard-to-transfect goat mammary epithelia cells. The results of the microscopy analysis revealed that transfected DNA entered the nucleus via the nuclear pores, facilitated by NLS. Analysis of the cell cycle demonstrated that the cytotoxic effects of NLS and SPB-NLS were low. In conclusion, the results of the present study demonstrate that SPB-NLS acts as a transfection-enhancing agent and may be used both to enhance nuclear delivery and for the development of genetically modified animals." @default.
• W2021573022 created "2016-06-24" @default.
• W2021573022 creator A5015617182 @default.
• W2021573022 creator A5039837606 @default.
• W2021573022 creator A5040242042 @default.
• W2021573022 creator A5079555574 @default.
• W2021573022 creator A5082860873 @default.
• W2021573022 creator A5089477321 @default.
• W2021573022 date "2014-04-08" @default.
• W2021573022 modified "2023-10-10" @default.
• W2021573022 title "Enhancement of transfection efficiency with NLS and SPB-NLS" @default.
• W2021573022 cites W1508758974 @default.
• W2021573022 cites W1549673200 @default.
• W2021573022 cites W1977502221 @default.
• W2021573022 cites W1977936350 @default.
• W2021573022 cites W1978372522 @default.
• W2021573022 cites W1992909684 @default.
• W2021573022 cites W1993471191 @default.
• W2021573022 cites W1995953841 @default.
• W2021573022 cites W1999292877 @default.
• W2021573022 cites W2004281525 @default.
• W2021573022 cites W2008657214 @default.
• W2021573022 cites W2008844864 @default.
• W2021573022 cites W2014639173 @default.
• W2021573022 cites W2020199268 @default.
• W2021573022 cites W2048310708 @default.
• W2021573022 cites W2048432868 @default.
• W2021573022 cites W2051790918 @default.
• W2021573022 cites W2056736337 @default.
• W2021573022 cites W2074702806 @default.
• W2021573022 cites W2081076598 @default.
• W2021573022 cites W2083756890 @default.
• W2021573022 cites W2087893222 @default.
• W2021573022 cites W2089132016 @default.
• W2021573022 cites W2105307577 @default.
• W2021573022 cites W2113019183 @default.
• W2021573022 cites W2114292940 @default.
• W2021573022 cites W2134934522 @default.
• W2021573022 cites W2150574768 @default.
• W2021573022 cites W2164114035 @default.
• W2021573022 cites W54353042 @default.
• W2021573022 doi "https://doi.org/10.3892/mmr.2014.2121" @default.
• W2021573022 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24715001" @default.
• W2021573022 hasPublicationYear "2014" @default.
• W2021573022 type Work @default.
• W2021573022 sameAs 2021573022 @default.
• W2021573022 citedByCount "0" @default.
• W2021573022 crossrefType "journal-article" @default.
• W2021573022 hasAuthorship W2021573022A5015617182 @default.
• W2021573022 hasAuthorship W2021573022A5039837606 @default.
• W2021573022 hasAuthorship W2021573022A5040242042 @default.
• W2021573022 hasAuthorship W2021573022A5079555574 @default.
• W2021573022 hasAuthorship W2021573022A5082860873 @default.
• W2021573022 hasAuthorship W2021573022A5089477321 @default.
• W2021573022 hasBestOaLocation W20215730221 @default.
• W2021573022 hasConcept C104317684 @default.
• W2021573022 hasConcept C142613039 @default.
• W2021573022 hasConcept C153911025 @default.
• W2021573022 hasConcept C186435350 @default.
• W2021573022 hasConcept C2780114586 @default.
• W2021573022 hasConcept C2780723820 @default.
• W2021573022 hasConcept C49805395 @default.
• W2021573022 hasConcept C54009773 @default.
• W2021573022 hasConcept C54355233 @default.
• W2021573022 hasConcept C55493867 @default.
• W2021573022 hasConcept C59157529 @default.
• W2021573022 hasConcept C81885089 @default.
• W2021573022 hasConcept C86803240 @default.
• W2021573022 hasConcept C95444343 @default.
• W2021573022 hasConceptScore W2021573022C104317684 @default.
• W2021573022 hasConceptScore W2021573022C142613039 @default.
• W2021573022 hasConceptScore W2021573022C153911025 @default.
• W2021573022 hasConceptScore W2021573022C186435350 @default.
• W2021573022 hasConceptScore W2021573022C2780114586 @default.
• W2021573022 hasConceptScore W2021573022C2780723820 @default.
• W2021573022 hasConceptScore W2021573022C49805395 @default.
• W2021573022 hasConceptScore W2021573022C54009773 @default.
• W2021573022 hasConceptScore W2021573022C54355233 @default.
• W2021573022 hasConceptScore W2021573022C55493867 @default.
• W2021573022 hasConceptScore W2021573022C59157529 @default.
• W2021573022 hasConceptScore W2021573022C81885089 @default.
• W2021573022 hasConceptScore W2021573022C86803240 @default.
• W2021573022 hasConceptScore W2021573022C95444343 @default.
• W2021573022 hasLocation W20215730221 @default.
• W2021573022 hasLocation W20215730222 @default.
• W2021573022 hasOpenAccess W2021573022 @default.
• W2021573022 hasPrimaryLocation W20215730221 @default.
• W2021573022 hasRelatedWork W1975678077 @default.
• W2021573022 hasRelatedWork W2003702457 @default.
• W2021573022 hasRelatedWork W2040933678 @default.
• W2021573022 hasRelatedWork W2118477548 @default.
• W2021573022 hasRelatedWork W2357995729 @default.
• W2021573022 hasRelatedWork W2361789318 @default.
• W2021573022 hasRelatedWork W2362439445 @default.
• W2021573022 hasRelatedWork W2369654325 @default.
• W2021573022 hasRelatedWork W2379485080 @default.
• W2021573022 hasRelatedWork W2503746655 @default.
• W2021573022 isParatext "false" @default.
• W2021573022 isRetracted "false" @default.
• W2021573022 magId "2021573022" @default.

• next